Title |
BID‐dependent release of mitochondrial SMAC dampens XIAP‐mediated immunity against Shigella
|
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Published in |
EMBO Journal, July 2014
|
DOI | 10.15252/embj.201387244 |
Pubmed ID | |
Authors |
Maria Andree, Jens M Seeger, Stephan Schüll, Oliver Coutelle, Diana Wagner-Stippich, Katja Wiegmann, Claudia M Wunderlich, Kerstin Brinkmann, Pia Broxtermann, Axel Witt, Melanie Fritsch, Paola Martinelli, Harald Bielig, Tobias Lamkemeyer, Elena I Rugarli, Thomas Kaufmann, Anja Sterner-Kock, F Thomas Wunderlich, Andreas Villunger, L Miguel Martins, Martin Krönke, Thomas A Kufer, Olaf Utermöhlen, Hamid Kashkar |
Abstract |
The X-linked inhibitor of apoptosis protein (XIAP) is a potent caspase inhibitor, best known for its anti-apoptotic function in cancer. During apoptosis, XIAP is antagonized by SMAC, which is released from the mitochondria upon caspase-mediated activation of BID. Recent studies suggest that XIAP is involved in immune signaling. Here, we explore XIAP as an important mediator of an immune response against the enteroinvasive bacterium Shigella flexneri, both in vitro and in vivo. Our data demonstrate for the first time that Shigella evades the XIAP-mediated immune response by inducing the BID-dependent release of SMAC from the mitochondria. Unlike apoptotic stimuli, Shigella activates the calpain-dependent cleavage of BID to trigger the release of SMAC, which antagonizes the inflammatory action of XIAP without inducing apoptosis. Our results demonstrate how the cellular death machinery can be subverted by an invasive pathogen to ensure bacterial colonization. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Norway | 1 | 1% |
Unknown | 82 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 24 | 29% |
Researcher | 17 | 20% |
Student > Bachelor | 9 | 11% |
Student > Master | 6 | 7% |
Other | 4 | 5% |
Other | 11 | 13% |
Unknown | 12 | 14% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 28 | 34% |
Biochemistry, Genetics and Molecular Biology | 25 | 30% |
Immunology and Microbiology | 11 | 13% |
Medicine and Dentistry | 5 | 6% |
Business, Management and Accounting | 1 | 1% |
Other | 2 | 2% |
Unknown | 11 | 13% |