↓ Skip to main content

Lectin Methods and Protocols

Overview of attention for book
Cover of 'Lectin Methods and Protocols'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter -896033958 Light Microscopy
  3. Altmetric Badge
    Chapter -896033940 Lectin Histochemistry and Cytochemistry—Light Microscopy
  4. Altmetric Badge
    Chapter -896033920 Lectin—Gold Histochemistry on Paraffin and Lowicryl K4M Sections Using Biotin and Digoxigenin-Conjugated Lectins
  5. Altmetric Badge
    Chapter -896033906 Use of Fluorochrome-Labeled Lectins in Light Microscopy
  6. Altmetric Badge
    Chapter -896033896 The Use of Lectins in Combination with Enzymatic Digestion for the Study of Glycoconjugates in Cartilage
  7. Altmetric Badge
    Chapter -896033888 Applications of Lectin Histochemistry and Cytochemistry in Diagnosis and Prognosis
  8. Altmetric Badge
    Chapter -896033864 Electron Microscopy
  9. Altmetric Badge
    Chapter -896033850 Electron Microscopy
  10. Altmetric Badge
    Chapter -896033840 Amplification of Lectin-Gold Histochemistry
  11. Altmetric Badge
    Chapter -896033828 Electron Microscopic Methods for the Demonstration of Lectin-Binding Sites in Cancer Cell Lines
  12. Altmetric Badge
    Chapter -896033814 Application of Sequential Smith Degradation to Lectin Blots
  13. Altmetric Badge
    Chapter -896033802 Blot Analysis with Lectins for the Evaluation of Glycoproteins in Cultured Cells and Tissues
  14. Altmetric Badge
    Chapter -896033794 Characterization of HIV gp120 Envelope Glycoprotein by Lectin Analysis
  15. Altmetric Badge
    Chapter -896033786 Use of Lectins for Characterization of O-Linked Glycans of Herpes Simplex Virus Glycoproteins
  16. Altmetric Badge
    Chapter -896033766 Use of Lectin for Detection of Agalactosyl IgG
  17. Altmetric Badge
    Chapter -896033754 Lectins for Detection of Altered Glycosylation of Circulating Glycoproteins
  18. Altmetric Badge
    Chapter -896033746 Detection of Altered Glycosylation of α-Fetoprotein Using Lectin-Affinity Electrophoresis
  19. Altmetric Badge
    Chapter -896033734 Use of Lectin-Affinity Electrophoresis for Quantification and Characterization of Glycoforms of α-1 Acid Glycoprotein
  20. Altmetric Badge
    Chapter -896033726 ABO(H) Blood Group Expression on Circulating Glycoproteins
  21. Altmetric Badge
    Chapter -896033712 Lectin/Antibody “Sandwich” ELISA for Quantification of Circulating Mucin as a Diagnostic Test for Pancreatic Cancer
  22. Altmetric Badge
    Chapter -896033706 Quantification of Intestinal Mucins
  23. Altmetric Badge
    Chapter -896033696 Purification and Characterization of Human Serum and Secretory IgA1 and IgA2 Using Jacalin
  24. Altmetric Badge
    Chapter -896033682 Use of Lectins in Affinity Purification of HIV and SIV Envelope Glycoproteins
  25. Altmetric Badge
    Chapter -896033670 T-Cell Receptor Purification
  26. Altmetric Badge
    Chapter -896033660 Use of Monomeric, Monovalent Lectin Derivatives for Flow Cytometric Analysis of Cell Surface Glycoconjugates
  27. Altmetric Badge
    Chapter -896033654 Analysis of Subcellular Components by Fluorescent-Lectin Binding and Flow Cytometry
  28. Altmetric Badge
    Chapter -896033642 Lectins as Tools for the Purification of Liver Endothelial Cells
  29. Altmetric Badge
    Chapter -896033632 The Use of Soybean Agglutinin (SBA) for Bone Marrow (BM) Purging and Hematopoietic Progenitor Cell Enrichment in Clinical Bone Marrow Transplantation
  30. Altmetric Badge
    Chapter -896033610 Combined Lectin/Monoclonal Antibody Purging of Bone Marrow for Use in Conjunction with Autologous Bone Marrow Transplantation in the Treatment of Multiple Myeloma
  31. Altmetric Badge
    Chapter -896033596 Mechanisms and Assessment of Mitogenesis
  32. Altmetric Badge
    Chapter -896033582 Mitogenic Effects of Lectins on Epithelial Cells
  33. Altmetric Badge
    Chapter -896033576 Use of Lectins as Mitogens for Lymphocytes
  34. Altmetric Badge
    Chapter -896033568 Effect of Lectins on Uptake of Polyamines
  35. Altmetric Badge
    Chapter -896033554 Effects of Lectins on Cytoskeletal Organization in Mammalian Cells
  36. Altmetric Badge
    Chapter -896033538 Effect of Lectins on Protein Kinase Activity
  37. Altmetric Badge
    Chapter -896033528 Lectin-Induced Calcium Mobilization in Human Platelets
  38. Altmetric Badge
    Chapter -896033520 Lectin-Triggered Superoxide/H2O2 and Granule Enzyme Release from Cells
  39. Altmetric Badge
    Chapter -896033508 Cytotoxic Effects of Lectins
  40. Altmetric Badge
    Chapter -896033500 Application of the Lectin-Dependent Cell-Mediated Cytotoxicity Assay to Bronchoalveolar Lavage Fluid and Venous Blood Samples Collected from Canine Lung Allografts
  41. Altmetric Badge
    Chapter -896033486 The Effect of Lectins on Crypt Cell Proliferation in Organ Culture
  42. Altmetric Badge
    Chapter -896033476 Effects of Lectin Ingestion on Animal Growth and Internal Organs
  43. Altmetric Badge
    Chapter -896033466 Lectin Ingestion
  44. Altmetric Badge
    Chapter -896033456 Assessment of lectin inactivation by heat and digestion.
  45. Altmetric Badge
    Chapter -896033444 Use of Lectins as Transganglionic Neuronal Tracers in the Study of Unmyelinated Primary Sensory Neurons
  46. Altmetric Badge
    Chapter -896033432 Lectin Inhibition of Bacterial Adhesion to Animal Cells
  47. Altmetric Badge
    Chapter -896033422 Inhibition of HIV Infection by Lectin Binding to CD4
  48. Altmetric Badge
    Chapter -896033406 Inhibition of HIV Infection by Lectin Binding to gp120
  49. Altmetric Badge
    Chapter -896033394 Absorption Enhancement by Lectin-Mediated Endo- and Transcytosis
  50. Altmetric Badge
    Chapter -896033378 The Use of Lectins for Liposome Targeting in Drug Delivery
Attention for Chapter -896033456: Assessment of lectin inactivation by heat and digestion.
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (92nd percentile)

Mentioned by

blogs
1 blog
twitter
7 tweeters
facebook
2 Facebook pages
video
6 video uploaders

Citations

dimensions_citation
17 Dimensions

Readers on

mendeley
38 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Assessment of lectin inactivation by heat and digestion.
Chapter number -896033456
Book title
Lectin Methods and Protocols
Published in
Methods in molecular medicine, January 1998
DOI 10.1385/0-89603-396-1:505
Pubmed ID
Book ISBNs
978-0-89603-396-2, 978-1-59259-593-8
Authors

A Pusztai, G Grant, Arpad Pusztai, George Grant, Pusztai, A, Grant, G

Editors

Jonathan M. Rhodes, Jeremy D. Milton

Abstract

Proteins/glycoproteins from plants, particularly lectins, are more resistant to heat denaturation than animal proteins (1, 2). With legume seeds, whose lectin content is appreciable, this presents potentially serious problems in nutritional practice. Therefore, before they can be used safely, legume-based food/ feeds usually require thorough and expensive heat processing to inactivate antinutritive components. Indeed, dry or moist heating of seeds at 70°C for several h has little or no effect on their lectin activity (Fig. 1) and treatment at much higher temperatures is needed to inactivate the biological and antinutritional effects of legume lectins (1, 2). The safety aspect is even more serious with some monocot lectins, such as wheatgerm agglutinin or a number of oilseed lectins, such as peanut agglutinin and many others because they are extremely heat stable and normal cooking or other conventional heat treatments may fail to inactivate them (3) Thus, the best way to avoid potential harmful effects of these heat-resistant lectins is to limit their dietary intake to a minimum. Fig. 1. Loss of lectin activity during aqueous heat treatment of soybean at various temperatures.

Twitter Demographics

The data shown below were collected from the profiles of 7 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 3%
Poland 1 3%
Belgium 1 3%
Unknown 35 92%

Demographic breakdown

Readers by professional status Count As %
Student > Master 10 26%
Researcher 8 21%
Student > Bachelor 5 13%
Other 3 8%
Student > Doctoral Student 2 5%
Other 5 13%
Unknown 5 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 32%
Biochemistry, Genetics and Molecular Biology 7 18%
Medicine and Dentistry 3 8%
Chemistry 3 8%
Psychology 1 3%
Other 4 11%
Unknown 8 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 August 2020.
All research outputs
#1,261,579
of 17,608,563 outputs
Outputs from Methods in molecular medicine
#1
of 39 outputs
Outputs of similar age
#15,863
of 199,011 outputs
Outputs of similar age from Methods in molecular medicine
#1
of 1 outputs
Altmetric has tracked 17,608,563 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 39 research outputs from this source. They receive a mean Attention Score of 3.0. This one scored the same or higher as 38 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 199,011 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them