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Isolation and characterization of porcine epidemic diarrhea virus associated with the 2014 disease outbreak in Mexico: case report

Overview of attention for article published in BMC Veterinary Research, January 2016
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1 tweeter
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1 Facebook page

Citations

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14 Dimensions

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36 Mendeley
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Title
Isolation and characterization of porcine epidemic diarrhea virus associated with the 2014 disease outbreak in Mexico: case report
Published in
BMC Veterinary Research, January 2016
DOI 10.1186/s12917-016-0763-z
Pubmed ID
Authors

María Elena Trujillo-Ortega, Rolando Beltrán-Figueroa, Montserrat Elemi García-Hernández, Mireya Juárez-Ramírez, Alicia Sotomayor-González, Erika N. Hernández-Villegas, José F. Becerra-Hernández, Rosa Elena Sarmiento-Silva

Abstract

Interest in porcine epidemic diarrhea has grown since the 2013 outbreak in the United States caused major losses, with mortality rates up to 100 % in suckling piglets. In Mexico, an outbreak of porcine epidemic diarrhea, characterized by 100 % mortality in piglets, began in March 2014 in the State of Mexico. The aim of this study was to confirm and identify porcine epidemic diarrhea virus (PEDV) in samples from piglets with suggestive clinical signs using virological, histological, and molecular techniques. Necropsy was performed on 13 piglets from two litters with initial and advanced clinical signs. Suggestive lesions of acute infection with PEDV were detected in histological sections of the small and large bowels; specifically, multiple virus particles with visible crown-shaped projections were observed using electron microscopy and negative staining. Viral isolation was performed in Vero cells with trypsin. Infection was monitored by observation of cytopathic effect, and titration was determined by TCID50/ml. The presence of the PEDV in cultures and clinical samples was confirmed by RT-PCR amplification and sequencing of a 651-bp segment of the S glycoprotein gene, as well as a 681-bp matrix protein gene. The nucleotide sequence analysis of the Mexican isolates showed marked homology to viruses that circulated in 2013 in Colorado, USA. In this paper we confirm the isolation and characterization of PEDV from animals with early and advanced clinical signs.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 36 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 10 28%
Student > Master 10 28%
Researcher 4 11%
Lecturer 2 6%
Lecturer > Senior Lecturer 1 3%
Other 4 11%
Unknown 5 14%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 8 22%
Agricultural and Biological Sciences 7 19%
Biochemistry, Genetics and Molecular Biology 6 17%
Computer Science 2 6%
Unspecified 2 6%
Other 6 17%
Unknown 5 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 April 2017.
All research outputs
#7,150,057
of 9,705,997 outputs
Outputs from BMC Veterinary Research
#836
of 1,489 outputs
Outputs of similar age
#171,098
of 264,908 outputs
Outputs of similar age from BMC Veterinary Research
#36
of 84 outputs
Altmetric has tracked 9,705,997 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,489 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 34th percentile – i.e., 34% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,908 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 84 others from the same source and published within six weeks on either side of this one. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.