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Microarray-based genotyping and detection of drug-resistant HBV mutations from 620 Chinese patients with chronic HBV infection

Overview of attention for article published in The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases, May 2015
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Title
Microarray-based genotyping and detection of drug-resistant HBV mutations from 620 Chinese patients with chronic HBV infection
Published in
The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases, May 2015
DOI 10.1016/j.bjid.2015.03.012
Pubmed ID
Authors

Hua, Wenhao, Zhang, Guanbin, Guo, Shujun, Li, Weijie, Sun, Lanhua, Xiang, Guangxin

Abstract

Research has shown that hepatitis B virus (HBV) genotypes are closely linked to the clinical manifestations, treatment, and prognosis of the disease. To study the association between genotype and drug-resistant HBV mutations in 620 Chinese patients with chronic HBV infection. HBV DNA levels were determined using real-time quantitative PCR in plasma samples. Microarrays were performed for the simultaneous detection of HBV genotypes (HBV/B, C, and D) and drug-resistance-related hotspot mutations. A portion of the samples analyzed using microarrays was selected randomly and the data were confirmed using direct DNA sequencing. Most samples were genotype C (471/620; 76.0%), followed by genotype B (149/620; 24.0%). Among the 620 patient samples, 17 (2.7%) had nucleotide analogs (NA) resistance-related mutations. Of these, nine and eight patients carried lamivudine (LAM)-/telbivudine (LdT)-resistance mutations (rtL180M, rtM204I/V) and adefovir (ADV)-resistance mutations (rtA181T/V, rtN236T), respectively. No patients had both LAM- and either ADV or entecavir (ETV) resistance mutations. Additionally, out of the 620 patient samples, 64.0% (397/620) were also detected with the precore stop-codon mutation (G1896A) by microarray assay. The results of the current study revealed that the prevalence of NA-resistance in Chinese hospitalized HBV-positive patients was so low that intensive NA-resistance testing before nucleotide analog (NA) treatment might not be required. In addition, the present study suggests that chronic HBV patients with genotype C were infected with fitter viruses and had an increased prevalence of NA-resistance mutations compared to genotype B virus.

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Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 4%
Unknown 22 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 26%
Student > Postgraduate 4 17%
Student > Bachelor 2 9%
Other 2 9%
Student > Master 2 9%
Other 1 4%
Unknown 6 26%
Readers by discipline Count As %
Medicine and Dentistry 5 22%
Biochemistry, Genetics and Molecular Biology 4 17%
Agricultural and Biological Sciences 4 17%
Nursing and Health Professions 1 4%
Environmental Science 1 4%
Other 2 9%
Unknown 6 26%