Chapter title |
Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts into Induced Pluripotent Stem Cells (iPSCs).
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Chapter number | 198 |
Book title |
Induced Pluripotent Stem (iPS) Cells
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Published in |
Methods in molecular biology, February 2015
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DOI | 10.1007/7651_2015_198 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3054-8, 978-1-4939-3055-5
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Authors |
Lucas V Greder, Jason Post, James R Dutton, Lucas V. Greder, James R. Dutton, Greder, Lucas V., Post, Jason, Dutton, James R. |
Editors |
Kursad Turksen, Andras Nagy |
Abstract |
The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) using a combination of defined transcription factors has become one of the most widely used techniques in stem cell biology. A critical, early event in iPSC reprogramming is the induction of the endogenous transcription factor network that maintains pluripotency in iPSCs. Here we describe using a transgenic, conditional Oct4-Cre construct to investigate the spatial and temporal induction of endogenous Oct4 expression during the reprogramming of mouse fibroblasts into iPS cells. |
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