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Comparison of different protocols for the extraction of microbial DNA from reef corals

Overview of attention for article published in Brazilian Journal of Microbiology, April 2012
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Title
Comparison of different protocols for the extraction of microbial DNA from reef corals
Published in
Brazilian Journal of Microbiology, April 2012
DOI 10.1590/s1517-83822012000200012
Pubmed ID
Authors

Santos, H F, Carmo, F L, Leite, D C A, Jesus, H E, Maalouf, P De Carvalho, Almeida, C, Soriano, A U, Altomari, D, Suhett, L, Vólaro, V, Valoni, E, Francisco, M, Vieira, J, Rocha, R, Sardinha, B L, Mendes, L B, João, R R, Lacava, B, Jesus, R F, Sebastian, G V, Pessoa, A, van Elsas, J D, Rezende, R P, Pires, D O, Duarte, G, Castro, C B, Rosado, A S, Peixoto, R S

Abstract

This study aimed to test different protocols for the extraction of microbial DNA from the coral Mussismilia harttii. Four different commercial kits were tested, three of them based on methods for DNA extraction from soil (FastDNA SPIN Kit for soil, MP Bio, PowerSoil DNA Isolation Kit, MoBio, and ZR Soil Microbe DNA Kit, Zymo Research) and one kit for DNA extraction from plants (UltraClean Plant DNA Isolation Kit, MoBio). Five polyps of the same colony of M. harttii were macerated and aliquots were submitted to DNA extraction by the different kits. After extraction, the DNA was quantified and PCR-DGGE was used to study the molecular fingerprint of Bacteria and Eukarya. Among the four kits tested, the ZR Soil Microbe DNA Kit was the most efficient with respect to the amount of DNA extracted, yielding about three times more DNA than the other kits. Also, we observed a higher number and intensities of DGGE bands for both Bacteria and Eukarya with the same kit. Considering these results, we suggested that the ZR Soil Microbe DNA Kit is the best adapted for the study of the microbial communities of corals.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 85 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 2 2%
India 1 1%
France 1 1%
New Zealand 1 1%
Unknown 80 94%

Demographic breakdown

Readers by professional status Count As %
Student > Master 19 22%
Student > Ph. D. Student 16 19%
Researcher 12 14%
Student > Bachelor 11 13%
Professor > Associate Professor 4 5%
Other 15 18%
Unknown 8 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 40 47%
Environmental Science 10 12%
Biochemistry, Genetics and Molecular Biology 9 11%
Immunology and Microbiology 3 4%
Engineering 2 2%
Other 5 6%
Unknown 16 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 August 2016.
All research outputs
#4,317,489
of 8,150,076 outputs
Outputs from Brazilian Journal of Microbiology
#67
of 185 outputs
Outputs of similar age
#141,628
of 257,748 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#4
of 4 outputs
Altmetric has tracked 8,150,076 research outputs across all sources so far. This one is in the 27th percentile – i.e., 27% of other outputs scored the same or lower than it.
So far Altmetric has tracked 185 research outputs from this source. They receive a mean Attention Score of 1.9. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 257,748 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one.