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Analysis and Purification of Mouse Intestinal Dendritic Cell and Macrophage Subsets by Flow Cytometry

Overview of attention for article published in Current protocols in immunology, August 2016
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Title
Analysis and Purification of Mouse Intestinal Dendritic Cell and Macrophage Subsets by Flow Cytometry
Published in
Current protocols in immunology, August 2016
DOI 10.1002/cpim.11
Pubmed ID
Authors

Balázs Koscsó, Milena Bogunovic

Abstract

The unit presents a method for analysis of intestinal dendritic cell (DC) and macrophage subsets by flow cytometry in the single cell suspension prepared from the mouse small and large intestine (Basic Protocol). describes a strategy to enrich the hematopoietic cell fraction in the sample by Percoll gradient centrifugation, and describes preparation of single cell suspensions from specific tissue layers of the small intestine, such as the epithelium, villi mucosa, submucosa, and muscularis externa. Finally, Support Protocol explains how to purify specific intestinal DC and macrophage subsets by flow-cytometry-based cell sorting. © 2016 by John Wiley & Sons, Inc.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 2%
Unknown 43 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 25%
Student > Doctoral Student 5 11%
Student > Ph. D. Student 5 11%
Student > Master 4 9%
Lecturer 2 5%
Other 3 7%
Unknown 14 32%
Readers by discipline Count As %
Immunology and Microbiology 10 23%
Agricultural and Biological Sciences 8 18%
Biochemistry, Genetics and Molecular Biology 6 14%
Medicine and Dentistry 3 7%
Pharmacology, Toxicology and Pharmaceutical Science 2 5%
Other 2 5%
Unknown 13 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2016.
All research outputs
#22,756,649
of 25,371,288 outputs
Outputs from Current protocols in immunology
#157
of 182 outputs
Outputs of similar age
#339,042
of 381,020 outputs
Outputs of similar age from Current protocols in immunology
#2
of 2 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 182 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 381,020 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one.