Title |
Identification of essential genes of the periodontal pathogen Porphyromonas gingivalis
|
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Published in |
BMC Genomics, October 2012
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DOI | 10.1186/1471-2164-13-578 |
Pubmed ID | |
Authors |
Brian A Klein, Elizabeth L Tenorio, David W Lazinski, Andrew Camilli, Margaret J Duncan, Linden T Hu |
Abstract |
Porphyromonas gingivalis is a Gram-negative anaerobic bacterium associated with periodontal disease onset and progression. Genetic tools for the manipulation of bacterial genomes allow for in-depth mechanistic studies of metabolism, physiology, interspecies and host-pathogen interactions. Analysis of the essential genes, protein-coding sequences necessary for survival of P. gingivalis by transposon mutagenesis has not previously been attempted due to the limitations of available transposon systems for the organism. We adapted a Mariner transposon system for mutagenesis of P. gingivalis and created an insertion mutant library. By analyzing the location of insertions using massively-parallel sequencing technology we used this mutant library to define genes essential for P. gingivalis survival under in vitro conditions. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 33% |
Canada | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 33% |
Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Members of the public | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 1% |
Australia | 1 | <1% |
India | 1 | <1% |
Brazil | 1 | <1% |
Mexico | 1 | <1% |
United Kingdom | 1 | <1% |
Unknown | 165 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 49 | 28% |
Researcher | 36 | 21% |
Student > Master | 21 | 12% |
Student > Bachelor | 11 | 6% |
Student > Doctoral Student | 7 | 4% |
Other | 21 | 12% |
Unknown | 27 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 60 | 35% |
Biochemistry, Genetics and Molecular Biology | 29 | 17% |
Immunology and Microbiology | 20 | 12% |
Medicine and Dentistry | 15 | 9% |
Chemical Engineering | 4 | 2% |
Other | 12 | 7% |
Unknown | 32 | 19% |