| Chapter title |
Clickable Polymeric Coating for Oriented Peptide Immobilization.
|
|---|---|
| Chapter number | 13 |
| Book title |
Peptide Microarrays
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3037-1_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3036-4, 978-1-4939-3037-1
|
| Authors |
Laura Sola, Alessandro Gori, Marina Cretich, Chiara Finetti, Caterina Zilio, Marcella Chiari |
| Editors |
Marina Cretich, Marcella Chiari |
| Abstract |
A new methodology for the fabrication of an high-performance peptide microarray is reported, combining the higher sensitivity of a layered Si-SiO2 substrate with the oriented immobilization of peptides using a N,N-dimethylacrylamide-based polymeric coating that contains alkyne monomers as functional groups. This clickable polymer allows the oriented attachment of azido-modified peptides via a copper-mediated azide/alkyne cycloaddition. A similar coating that does not contain the alkyne functionality has been used as comparison, to demonstrate the importance of a proper orientation for facilitating the probe recognition and interaction with the target antibody. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 50% |
| Unknown | 3 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 2 | 33% |
| Unknown | 4 | 67% |