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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
|
|---|---|
| Published in |
Brazilian Journal of Microbiology, July 2016
|
| DOI | 10.1016/j.bjm.2016.07.022 |
| Pubmed ID | |
| Authors |
Sandra Arenhart, José Valter Joaquim Silva, Eduardo Furtado Flores, Rudi Weiblen, Laura Helena Vega Gonzales Gil |
| Abstract |
The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2 and chi-NADL/IBSP4ncp#3). The recombinant clones were successfully recovered, resulting in viable viruses, having the kinetics of replication, focus size, and morphology similar to those of the parental virus, IBSP4ncp. In addition, the chimeric viruses remained stable for at least 10 passages in cell culture, maintaining their replication efficiency unaltered. Nucleotide sequencing revealed a few point mutations; nevertheless, the phenotype of the rescued viruses was nearly identical to that of the parental virus in all experiments. Thus, genetic stability of the chimeric clones and their phenotypic similarity to the parental virus confirm the ability of the yeast-based homologous recombination to maintain characteristics of the parental virus from which the recombinant viruses were derived. The data also support possible use of the yeast system for the manipulation of the BVDV genome. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 4% |
| Unknown | 27 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 7 | 25% |
| Student > Master | 4 | 14% |
| Researcher | 2 | 7% |
| Student > Ph. D. Student | 2 | 7% |
| Student > Doctoral Student | 1 | 4% |
| Other | 4 | 14% |
| Unknown | 8 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 29% |
| Veterinary Science and Veterinary Medicine | 5 | 18% |
| Biochemistry, Genetics and Molecular Biology | 4 | 14% |
| Medicine and Dentistry | 2 | 7% |
| Unknown | 9 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 January 2022.
All research outputs
#7,047,316
of 25,373,627 outputs
Outputs from Brazilian Journal of Microbiology
#145
of 1,377 outputs
Outputs of similar age
#112,496
of 380,103 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#3
of 20 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 1,377 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 380,103 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.