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Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones

Overview of attention for article published in Brazilian Journal of Microbiology, October 2016
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Title
Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
Published in
Brazilian Journal of Microbiology, October 2016
DOI 10.1016/j.bjm.2016.07.022
Pubmed ID
Authors

Sandra Arenhart, José Valter Joaquim Silva, Eduardo Furtado Flores, Rudi Weiblen, Laura Helena Vega Gonzales Gil

Abstract

The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2 and chi-NADL/IBSP4ncp#3). The recombinant clones were successfully recovered, resulting in viable viruses, having the kinetics of replication, focus size, and morphology similar to those of the parental virus, IBSP4ncp. In addition, the chimeric viruses remained stable for at least 10 passages in cell culture, maintaining their replication efficiency unaltered. Nucleotide sequencing revealed a few point mutations; nevertheless, the phenotype of the rescued viruses was nearly identical to that of the parental virus in all experiments. Thus, genetic stability of the chimeric clones and their phenotypic similarity to the parental virus confirm the ability of the yeast-based homologous recombination to maintain characteristics of the parental virus from which the recombinant viruses were derived. The data also support possible use of the yeast system for the manipulation of the BVDV genome.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 4%
Unknown 22 96%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 26%
Student > Master 4 17%
Student > Ph. D. Student 2 9%
Other 1 4%
Professor 1 4%
Other 4 17%
Unknown 5 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 35%
Veterinary Science and Veterinary Medicine 5 22%
Biochemistry, Genetics and Molecular Biology 3 13%
Medicine and Dentistry 2 9%
Unknown 5 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2016.
All research outputs
#10,978,493
of 12,355,257 outputs
Outputs from Brazilian Journal of Microbiology
#312
of 363 outputs
Outputs of similar age
#190,598
of 227,252 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#2
of 2 outputs
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So far Altmetric has tracked 363 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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