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Cell-free synthesis of functional phospholipase A1 from Serratia sp.

Overview of attention for article published in Biotechnology for Biofuels, July 2016
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Title
Cell-free synthesis of functional phospholipase A1 from Serratia sp.
Published in
Biotechnology for Biofuels, July 2016
DOI 10.1186/s13068-016-0563-5
Pubmed ID
Authors

Hye Jin Lim, Yu Jin Park, Yeon Jae Jang, Ji Eun Choi, Joon Young Oh, Ji Hyun Park, Jae Kwang Song, Dong-Myung Kim

Abstract

Phospholipase A1 is an enzyme that hydrolyzes phospholipids at the sn-1 position. It has potential applications across diverse fields including food, pharmaceutical, and biofuel industries. Although there has been increasing interest in the use of phospholipase A1 for degumming of plant oils during biodiesel production, production of recombinant phospholipase A1 has been hampered by low efficiency of gene expression and its toxicity to the host cell. While expression of phospholipase A1 in Escherichia coli resulted in extremely low productivity associated with inhibition of transformed cell growth, drastically higher production of functional phospholipase A1 was achieved in a cell-free protein synthesis system where enzyme expression is decoupled from cell physiology. Compared with expression in E. coli, cell-free synthesis resulted in an over 1000-fold higher titer of functional phospholipase A1. Cell-free produced phospholipase A1 was also used for successfully degumming crude plant oil. We demonstrate successful production of Serratia sp. phospholipase A1 in a cell-free protein synthesis system. Including the phospholipase A1 investigated in this study, many industrial enzymes can interfere with the regular physiology of cells, making cellular production of them problematic. With the experimental results presented herewith, we believe that cell-free protein synthesis will provide a viable option for rapid production of important industrial biocatalysts.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 17%
Student > Ph. D. Student 3 17%
Professor 3 17%
Student > Bachelor 2 11%
Lecturer 1 6%
Other 4 22%
Unknown 2 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 33%
Agricultural and Biological Sciences 3 17%
Engineering 2 11%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Unspecified 1 6%
Other 3 17%
Unknown 2 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 August 2016.
All research outputs
#7,121,735
of 8,229,276 outputs
Outputs from Biotechnology for Biofuels
#576
of 672 outputs
Outputs of similar age
#214,104
of 252,986 outputs
Outputs of similar age from Biotechnology for Biofuels
#19
of 19 outputs
Altmetric has tracked 8,229,276 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 672 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 252,986 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.