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Characterization and in Vitro Reaction Properties of 19 Unique Hairpin Telomeres from the Linear Plasmids of the Lyme Disease Spirochete

Overview of attention for article published in Journal of Biological Chemistry, January 2009
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (93rd percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

Mentioned by

blogs
2 blogs
twitter
7 tweeters
googleplus
1 Google+ user

Citations

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21 Dimensions

Readers on

mendeley
15 Mendeley
citeulike
1 CiteULike
Title
Characterization and in Vitro Reaction Properties of 19 Unique Hairpin Telomeres from the Linear Plasmids of the Lyme Disease Spirochete
Published in
Journal of Biological Chemistry, January 2009
DOI 10.1074/jbc.m808918200
Pubmed ID
Authors

Y. Tourand, J. Deneke, T. J. Moriarty, G. Chaconas, Yvonne Tourand, Jan Deneke, Tara J. Moriarty, George Chaconas

Abstract

The genome of the Lyme disease pathogen Borrelia burgdorferi contains about a dozen linear DNA molecules that carry covalently closed hairpin telomeres as a specialized mechanism for dealing with the end-replication problem. The hairpin telomeres are generated from replicative intermediates through a two-step transesterification promoted by the telomere resolvase ResT. Although the genome of B. burgdorferi has been sequenced, the sequence of most telomeres has remained unknown because of difficulties in recovering and completely sequencing the covalently closed hairpin ends. In this study we report a new approach for the direct sequencing Borrelia telomeres and report the sequence, characterization, and in vitro reaction properties of 19 unique telomeres. Surprisingly, a variation of greater than 160-fold in the initial reaction rates of in vitro ResT-mediated telomere resolution was observed between the most active and least active telomeres. Moreover, three of the hairpin telomeres were completely inactive in vitro, but their in vivo functionality was demonstrated. Our results provide important new information on the structure and function of the B. burgdorferi telomeres and suggest the possibility that factors besides the telomere resolvase ResT may influence the reaction in vivo and rescue those telomeres that are not functional in vitro with ResT alone.

Twitter Demographics

The data shown below were collected from the profiles of 7 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 27%
Researcher 4 27%
Other 3 20%
Professor 1 7%
Student > Doctoral Student 1 7%
Other 2 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 53%
Biochemistry, Genetics and Molecular Biology 4 27%
Immunology and Microbiology 2 13%
Philosophy 1 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 November 2018.
All research outputs
#754,650
of 12,184,206 outputs
Outputs from Journal of Biological Chemistry
#668
of 53,515 outputs
Outputs of similar age
#734,607
of 11,527,419 outputs
Outputs of similar age from Journal of Biological Chemistry
#667
of 53,351 outputs
Altmetric has tracked 12,184,206 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 53,515 research outputs from this source. They receive a mean Attention Score of 4.2. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 11,527,419 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 53,351 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.