Title |
Integrated miRNA, mRNA and protein expression analysis reveals the role of post-transcriptional regulation in controlling CHO cell growth rate
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Published in |
BMC Genomics, November 2012
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DOI | 10.1186/1471-2164-13-656 |
Pubmed ID | |
Authors |
Colin Clarke, Michael Henry, Padraig Doolan, Shane Kelly, Sinead Aherne, Noelia Sanchez, Paul Kelly, Paula Kinsella, Laura Breen, Stephen F Madden, Lin Zhang, Mark Leonard, Martin Clynes, Paula Meleady, Niall Barron |
Abstract |
To study the role of microRNA (miRNA) in the regulation of Chinese hamster ovary (CHO) cell growth, qPCR, microarray and quantitative LC-MS/MS analysis were utilised for simultaneous expression profiling of miRNA, mRNA and protein. The sample set under investigation consisted of clones with variable cellular growth rates derived from the same population. In addition to providing a systems level perspective on cell growth, the integration of multiple profiling datasets can facilitate the identification of non-seed miRNA targets, complement computational prediction tools and reduce false positive and false negative rates. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 1 | <1% |
Switzerland | 1 | <1% |
Ireland | 1 | <1% |
Italy | 1 | <1% |
Austria | 1 | <1% |
United Kingdom | 1 | <1% |
Canada | 1 | <1% |
Denmark | 1 | <1% |
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Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 42 | 29% |
Researcher | 41 | 28% |
Student > Master | 12 | 8% |
Professor > Associate Professor | 7 | 5% |
Student > Bachelor | 6 | 4% |
Other | 24 | 17% |
Unknown | 12 | 8% |
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Biochemistry, Genetics and Molecular Biology | 39 | 27% |
Computer Science | 5 | 3% |
Engineering | 4 | 3% |
Chemical Engineering | 4 | 3% |
Other | 14 | 10% |
Unknown | 18 | 13% |