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Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizing radiation

Overview of attention for article published in Stem Cell Research & Therapy, August 2016
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Title
Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizing radiation
Published in
Stem Cell Research & Therapy, August 2016
DOI 10.1186/s13287-016-0378-0
Pubmed ID
Authors

Woonhyeok Jeong, Xiao Yang, Jeongmi Lee, Youngwook Ryoo, Jinhee Kim, Youngkee Oh, Sunyoung Kwon, Dalie Liu, Daegu Son

Abstract

Adipose-derived stem cells (ASCs) are important to homeostasis and the regeneration of subcutaneous fat. Hence, we examined the proliferation and differentiation capacity of irradiated ASCs over time. Two female pigs received a single 18 Gy dose of ionizing radiation to an 18 × 8 cm area on the dorsal body skin via a 6 MeV electron beam. After irradiation, the ASCs were cultured from adipose tissue harvested from a non-irradiated area and an irradiated area at 2, 4, and 6 weeks. The proliferation capacity of ASCs was evaluated by a colony-forming units-fibroblasts (CFUs-Fs) assay, a cholecystokinin (CCK) test with 10 % fetal bovine serum (FBS), and a 1 % FBS culture test. The senescence of ASCs was evaluated through morphological examination, immunophenotyping, and β-galactosidase activity, and the multipotent differentiation potential of ASCs was evaluated in adipogenic, osteogenic, and chondrogenic differentiation media. Irradiated ASCs demonstrated significantly decreased proliferative capacity 6 weeks after irradiation. As well, the cells underwent senescence, which was confirmed by blunted morphology, weak mesenchymal cell surface marker expression, and elevated β-galactosidase activity. Irradiated ASCs also exhibited significant losses in the capacity for adipocyte and chondrocyte differentiation. In contrast, osteogenic differentiation was preserved in irradiated ASCs. We observed decreased proliferation and senescence of irradiated ASCs compared to non-irradiated ASCs 6 weeks after irradiation. Furthermore, irradiated ASCs demonstrated impaired adipocyte and chondrocyte differentiation but retained their osteogenic differentiation capacity. Our results could shed light on additional pathogenic effects of late irradiation, including subcutaneous fibrosis and calcinosis.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 5 20%
Other 4 16%
Student > Doctoral Student 3 12%
Student > Bachelor 2 8%
Student > Ph. D. Student 2 8%
Other 5 20%
Unknown 4 16%
Readers by discipline Count As %
Medicine and Dentistry 10 40%
Biochemistry, Genetics and Molecular Biology 4 16%
Agricultural and Biological Sciences 4 16%
Immunology and Microbiology 1 4%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Other 0 0%
Unknown 5 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2016.
All research outputs
#6,279,615
of 8,276,988 outputs
Outputs from Stem Cell Research & Therapy
#517
of 674 outputs
Outputs of similar age
#180,250
of 253,534 outputs
Outputs of similar age from Stem Cell Research & Therapy
#33
of 45 outputs
Altmetric has tracked 8,276,988 research outputs across all sources so far. This one is in the 13th percentile – i.e., 13% of other outputs scored the same or lower than it.
So far Altmetric has tracked 674 research outputs from this source. They receive a mean Attention Score of 5.0. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 253,534 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 45 others from the same source and published within six weeks on either side of this one. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.