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Characterization of a glucose-tolerant β-glucosidase from Anoxybacillus sp. DT3-1

Overview of attention for article published in Biotechnology for Biofuels, January 2016
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Title
Characterization of a glucose-tolerant β-glucosidase from Anoxybacillus sp. DT3-1
Published in
Biotechnology for Biofuels, January 2016
DOI 10.1186/s13068-016-0587-x
Pubmed ID
Authors

Chan, Chia Sing, Sin, Lee Li, Chan, Kok-Gan, Shamsir, Mohd Shahir, Manan, Fazilah Abd, Sani, Rajesh Kumar, Goh, Kian Mau

Abstract

In general, biofuel production involves biomass pretreatment and enzymatic saccharification, followed by the subsequent sugar conversion to biofuel via fermentation. The crucial step in the production of biofuel from biomass is the enzymatic saccharification. Many of the commercial cellulase enzyme cocktails, such as Spezyme(®) CP (Genencor), Acellerase™ 1000 (Genencor), and Celluclast(®) 1.5L (Novozymes), are ineffectively to release free glucose from the pretreated biomass without additional β-glucosidase. In this study, for the first time, a β-glucosidase DT-Bgl gene (1359 bp) was identified in the genome of Anoxybacillus sp. DT3-1, and cloned and heterologously expressed in Escherichia coli BL21. Phylogenetic analysis indicated that DT-Bgl belonged to glycosyl hydrolase (GH) family 1. The recombinant DT-Bgl was highly active on cello-oligosaccharides and p-nitrophenyl-β-d-glucopyranoside (pNPG). The DT-Bgl was purified using an Ni-NTA column, with molecular mass of 53 kDa using an SDS-PAGE analysis. It exhibited optimum activity at 70 °C and pH 8.5, and did not require any tested co-factors for activation. The K m and V max values for DT-Bgl were 0.22 mM and 923.7 U/mg, respectively, with pNPG as substrate. The DT-Bgl displayed high glucose tolerance, and retained 93 % activity in the presence of 10 M glucose. Anoxybacillus DT-Bgl is a novel thermostable β-glucosidase with low glucose inhibition, and converts long-chain cellodextrins to cellobiose, and further hydrolyse cellobiose to glucose. Results suggest that DT-Bgl could be useful in the development of a bioprocess for the efficient saccharification of lignocellulosic biomass.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 40 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 8 20%
Student > Bachelor 7 18%
Student > Ph. D. Student 7 18%
Unspecified 4 10%
Other 2 5%
Other 12 30%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 35%
Biochemistry, Genetics and Molecular Biology 12 30%
Unspecified 6 15%
Environmental Science 2 5%
Chemistry 2 5%
Other 4 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2016.
All research outputs
#7,157,761
of 8,276,988 outputs
Outputs from Biotechnology for Biofuels
#577
of 676 outputs
Outputs of similar age
#213,565
of 253,535 outputs
Outputs of similar age from Biotechnology for Biofuels
#19
of 20 outputs
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