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Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy

Overview of attention for article published in Journal of Venomous Animals and Toxins including Tropical Diseases, August 2016
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Title
Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
Published in
Journal of Venomous Animals and Toxins including Tropical Diseases, August 2016
DOI 10.1186/s40409-016-0078-3
Pubmed ID
Authors

Hai-long Duan, Qi-yi He, Bin Zhou, Wen-wen Wang, Bo Li, Ying-zheng Zhang, Qiu-ping Deng, Ying-feng Zhang, Xiao-dong Yu

Abstract

Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom antibody (immunoglobulin Y - IgY) from leghorn chickens. IgY in egg yolk from white leghorn chicken previously injected with T. albolabris venom was extracted by water, precipitated by ammonium sulfate and purified by affinity chromatographic system. IgY was identified by SDS-PAGE, ELISA and Western blot, and its neutralizing assay was conducted on mice. Chickens injected multiple times with T. albolabris venom elicited strong antibody responses, and from their egg yolk IgY was isolated and purified, which exhibited a single protein band on SDS-PAGE and two bands (about 65 and 35 kDa, respectively) under reduced conditions. Immunoblot analysis revealed that these IgY are polyclonal antibodies since they bind with most venom components. In the neutralizing assay, all mice survived while the ratios of IgY/venom reached up to 3.79 (50.0 mg/13.2 mg). IgY antibody response was successfully conducted in white leghorn chicken injected with T. albolabris venom. IgY against T. albolabris venom was obtained for the first time, and it exhibited strong neutralizing potency on mice. These results may lay a foundation for the development of IgY antivenom with clinical applications in the future.

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Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 27%
Student > Ph. D. Student 4 13%
Researcher 3 10%
Professor 1 3%
Student > Doctoral Student 1 3%
Other 4 13%
Unknown 9 30%
Readers by discipline Count As %
Immunology and Microbiology 5 17%
Biochemistry, Genetics and Molecular Biology 5 17%
Agricultural and Biological Sciences 4 13%
Veterinary Science and Veterinary Medicine 3 10%
Engineering 1 3%
Other 0 0%
Unknown 12 40%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 August 2016.
All research outputs
#9,759,232
of 15,918,484 outputs
Outputs from Journal of Venomous Animals and Toxins including Tropical Diseases
#192
of 391 outputs
Outputs of similar age
#140,922
of 267,163 outputs
Outputs of similar age from Journal of Venomous Animals and Toxins including Tropical Diseases
#1
of 1 outputs
Altmetric has tracked 15,918,484 research outputs across all sources so far. This one is in the 36th percentile – i.e., 36% of other outputs scored the same or lower than it.
So far Altmetric has tracked 391 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,163 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them