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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
|
|---|---|
| Published in |
Journal of Venomous Animals and Toxins including Tropical Diseases, August 2016
|
| DOI | 10.1186/s40409-016-0078-3 |
| Pubmed ID | |
| Authors |
Hai-long Duan, Qi-yi He, Bin Zhou, Wen-wen Wang, Bo Li, Ying-zheng Zhang, Qiu-ping Deng, Ying-feng Zhang, Xiao-dong Yu |
| Abstract |
Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom antibody (immunoglobulin Y - IgY) from leghorn chickens. IgY in egg yolk from white leghorn chicken previously injected with T. albolabris venom was extracted by water, precipitated by ammonium sulfate and purified by affinity chromatographic system. IgY was identified by SDS-PAGE, ELISA and Western blot, and its neutralizing assay was conducted on mice. Chickens injected multiple times with T. albolabris venom elicited strong antibody responses, and from their egg yolk IgY was isolated and purified, which exhibited a single protein band on SDS-PAGE and two bands (about 65 and 35 kDa, respectively) under reduced conditions. Immunoblot analysis revealed that these IgY are polyclonal antibodies since they bind with most venom components. In the neutralizing assay, all mice survived while the ratios of IgY/venom reached up to 3.79 (50.0 mg/13.2 mg). IgY antibody response was successfully conducted in white leghorn chicken injected with T. albolabris venom. IgY against T. albolabris venom was obtained for the first time, and it exhibited strong neutralizing potency on mice. These results may lay a foundation for the development of IgY antivenom with clinical applications in the future. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 45 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 13 | 29% |
| Student > Ph. D. Student | 4 | 9% |
| Researcher | 3 | 7% |
| Other | 2 | 4% |
| Student > Doctoral Student | 1 | 2% |
| Other | 4 | 9% |
| Unknown | 18 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 16% |
| Immunology and Microbiology | 5 | 11% |
| Biochemistry, Genetics and Molecular Biology | 5 | 11% |
| Veterinary Science and Veterinary Medicine | 3 | 7% |
| Chemistry | 2 | 4% |
| Other | 2 | 4% |
| Unknown | 21 | 47% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 August 2016.
All research outputs
#16,722,913
of 25,374,917 outputs
Outputs from Journal of Venomous Animals and Toxins including Tropical Diseases
#310
of 539 outputs
Outputs of similar age
#220,959
of 352,660 outputs
Outputs of similar age from Journal of Venomous Animals and Toxins including Tropical Diseases
#4
of 5 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 539 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,660 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one.