| Chapter title |
Identifying Bacterial Immune Evasion Proteins Using Phage Display.
|
|---|---|
| Chapter number | 4 |
| Book title |
Bacterial Pathogenesis
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6673-8_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6671-4, 978-1-4939-6673-8
|
| Authors |
Cindy Fevre, Lisette Scheepmaker, Pieter-Jan Haas |
| Editors |
Pontus Nordenfelt, Mattias Collin |
| Abstract |
Methods aimed at identification of immune evasion proteins are mainly rely on in silico prediction of sequence, structural homology to known evasion proteins or use a proteomics driven approach. Although proven successful these methods are limited by a low efficiency and or lack of functional identification. Here we describe a high-throughput genomic strategy to functionally identify bacterial immune evasion proteins using phage display technology. Genomic bacterial DNA is randomly fragmented and ligated into a phage display vector that is used to create a phage display library expressing bacterial secreted and membrane bound proteins. This library is used to select displayed bacterial secretome proteins that interact with host immune components. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 20% |
| Researcher | 2 | 20% |
| Student > Master | 2 | 20% |
| Student > Bachelor | 1 | 10% |
| Librarian | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 20% |
| Veterinary Science and Veterinary Medicine | 1 | 10% |
| Arts and Humanities | 1 | 10% |
| Computer Science | 1 | 10% |
| Immunology and Microbiology | 1 | 10% |
| Other | 1 | 10% |
| Unknown | 3 | 30% |