Chapter title |
Assessing Vacuolar Escape of Listeria Monocytogenes.
|
---|---|
Chapter number | 11 |
Book title |
Bacterial Pathogenesis
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6673-8_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6671-4, 978-1-4939-6673-8
|
Authors |
Juan J. Quereda, Martin Sachse, Damien Balestrino, Théodore Grenier, Jennifer Fredlund, Anne Danckaert, Nathalie Aulner, Spencer Shorte, Jost Enninga, Pascale Cossart, Javier Pizarro-Cerdá, Juan J Quereda |
Editors |
Pontus Nordenfelt, Mattias Collin |
Abstract |
Listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. Signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. In this chapter, we detail a microscopy FRET-based assay which allows quantitatively measuring L. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing L. monocytogenes. |
X Demographics
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Portugal | 1 | 6% |
Unknown | 17 | 94% |
Demographic breakdown
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Student > Ph. D. Student | 4 | 22% |
Student > Bachelor | 2 | 11% |
Student > Doctoral Student | 2 | 11% |
Researcher | 2 | 11% |
Professor | 2 | 11% |
Other | 4 | 22% |
Unknown | 2 | 11% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 4 | 22% |
Biochemistry, Genetics and Molecular Biology | 2 | 11% |
Business, Management and Accounting | 1 | 6% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Other | 2 | 11% |
Unknown | 2 | 11% |