Title |
Escherichia coli–expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen
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Published in |
BMC Infectious Diseases, November 2012
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DOI | 10.1186/1471-2334-12-325 |
Pubmed ID | |
Authors |
Sheikh M Talha, Satish Kumar Nemani, Teppo Salminen, Sushil Kumar, Sathyamangalam Swaminathan, Tero Soukka, Kim Pettersson, Navin Khanna |
Abstract |
The Human Immunodeficiency Virus type 1 (HIV-1) envelope glycoprotein gp160, useful in detecting anti-HIV-1 antibodies, is difficult to express in heterologous hosts. The major hurdles are its signal sequence, strong hydrophobic regions and heavy glycosylation. While it has not been possible to express full length recombinant (r)-gp160 in E. coli, it can be expressed in insect and mammalian cells, but at relatively higher cost. In this work, we report E. coli-based over-expression of r-gp160 variant and evaluate its performance in diagnostic immunoassays for the detection of anti-HIV-1 antibodies. |
X Demographics
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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India | 1 | 3% |
Unknown | 29 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 10 | 33% |
Student > Master | 6 | 20% |
Student > Ph. D. Student | 4 | 13% |
Student > Bachelor | 4 | 13% |
Other | 2 | 7% |
Other | 1 | 3% |
Unknown | 3 | 10% |
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Biochemistry, Genetics and Molecular Biology | 6 | 20% |
Agricultural and Biological Sciences | 4 | 13% |
Immunology and Microbiology | 3 | 10% |
Medicine and Dentistry | 3 | 10% |
Nursing and Health Professions | 2 | 7% |
Other | 6 | 20% |
Unknown | 6 | 20% |