↓ Skip to main content

Escherichia coli–expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen

Overview of attention for article published in BMC Infectious Diseases, November 2012
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
30 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Escherichia coli–expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen
Published in
BMC Infectious Diseases, November 2012
DOI 10.1186/1471-2334-12-325
Pubmed ID
Authors

Sheikh M Talha, Satish Kumar Nemani, Teppo Salminen, Sushil Kumar, Sathyamangalam Swaminathan, Tero Soukka, Kim Pettersson, Navin Khanna

Abstract

The Human Immunodeficiency Virus type 1 (HIV-1) envelope glycoprotein gp160, useful in detecting anti-HIV-1 antibodies, is difficult to express in heterologous hosts. The major hurdles are its signal sequence, strong hydrophobic regions and heavy glycosylation. While it has not been possible to express full length recombinant (r)-gp160 in E. coli, it can be expressed in insect and mammalian cells, but at relatively higher cost. In this work, we report E. coli-based over-expression of r-gp160 variant and evaluate its performance in diagnostic immunoassays for the detection of anti-HIV-1 antibodies.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 3%
Unknown 29 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 33%
Student > Master 6 20%
Student > Ph. D. Student 4 13%
Student > Bachelor 4 13%
Other 2 7%
Other 1 3%
Unknown 3 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 20%
Agricultural and Biological Sciences 4 13%
Immunology and Microbiology 3 10%
Medicine and Dentistry 3 10%
Nursing and Health Professions 2 7%
Other 6 20%
Unknown 6 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 November 2012.
All research outputs
#20,174,175
of 22,687,320 outputs
Outputs from BMC Infectious Diseases
#6,425
of 7,643 outputs
Outputs of similar age
#245,745
of 277,168 outputs
Outputs of similar age from BMC Infectious Diseases
#113
of 143 outputs
Altmetric has tracked 22,687,320 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,643 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,168 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 143 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.