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Real-Time High Resolution 3D Imaging of the Lyme Disease Spirochete Adhering to and Escaping from the Vasculature of a Living Host

Overview of attention for article published in PLoS Pathogens, June 2008
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (94th percentile)
  • High Attention Score compared to outputs of the same age and source (84th percentile)

Mentioned by

blogs
1 blog
twitter
23 tweeters
facebook
5 Facebook pages
video
1 video uploader

Citations

dimensions_citation
124 Dimensions

Readers on

mendeley
115 Mendeley
citeulike
1 CiteULike
connotea
1 Connotea
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Title
Real-Time High Resolution 3D Imaging of the Lyme Disease Spirochete Adhering to and Escaping from the Vasculature of a Living Host
Published in
PLoS Pathogens, June 2008
DOI 10.1371/journal.ppat.1000090
Pubmed ID
Authors

Tara J. Moriarty, M. Ursula Norman, Pina Colarusso, Troy Bankhead, Paul Kubes, George Chaconas

Abstract

Pathogenic spirochetes are bacteria that cause a number of emerging and re-emerging diseases worldwide, including syphilis, leptospirosis, relapsing fever, and Lyme borreliosis. They navigate efficiently through dense extracellular matrix and cross the blood-brain barrier by unknown mechanisms. Due to their slender morphology, spirochetes are difficult to visualize by standard light microscopy, impeding studies of their behavior in situ. We engineered a fluorescent infectious strain of Borrelia burgdorferi, the Lyme disease pathogen, which expressed green fluorescent protein (GFP). Real-time 3D and 4D quantitative analysis of fluorescent spirochete dissemination from the microvasculature of living mice at high resolution revealed that dissemination was a multi-stage process that included transient tethering-type associations, short-term dragging interactions, and stationary adhesion. Stationary adhesions and extravasating spirochetes were most commonly observed at endothelial junctions, and translational motility of spirochetes appeared to play an integral role in transendothelial migration. To our knowledge, this is the first report of high resolution 3D and 4D visualization of dissemination of a bacterial pathogen in a living mammalian host, and provides the first direct insight into spirochete dissemination in vivo.

Twitter Demographics

The data shown below were collected from the profiles of 23 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 115 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Hungary 2 2%
United Kingdom 2 2%
Sweden 1 <1%
Brazil 1 <1%
Netherlands 1 <1%
Uzbekistan 1 <1%
United States 1 <1%
Unknown 106 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 26 23%
Researcher 23 20%
Student > Master 15 13%
Student > Bachelor 12 10%
Professor > Associate Professor 8 7%
Other 20 17%
Unknown 11 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 45 39%
Medicine and Dentistry 17 15%
Biochemistry, Genetics and Molecular Biology 12 10%
Immunology and Microbiology 12 10%
Physics and Astronomy 4 3%
Other 11 10%
Unknown 14 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 22. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 January 2019.
All research outputs
#851,109
of 14,638,436 outputs
Outputs from PLoS Pathogens
#1,053
of 6,599 outputs
Outputs of similar age
#817,260
of 13,794,300 outputs
Outputs of similar age from PLoS Pathogens
#935
of 5,898 outputs
Altmetric has tracked 14,638,436 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 6,599 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 16.4. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 13,794,300 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 5,898 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.