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High levels of ephrinB2 over-expression increases the osteogenic differentiation of human mesenchymal stem cells and promotes enhanced cell mediated mineralisation in a polyethyleneimine-ephrinB2 gene…

Overview of attention for article published in Journal of Controlled Release, February 2013
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2 tweeters
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1 Facebook page

Citations

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39 Dimensions

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36 Mendeley
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Title
High levels of ephrinB2 over-expression increases the osteogenic differentiation of human mesenchymal stem cells and promotes enhanced cell mediated mineralisation in a polyethyleneimine-ephrinB2 gene-activated matrix
Published in
Journal of Controlled Release, February 2013
DOI 10.1016/j.jconrel.2012.11.013
Pubmed ID
Authors

Erica G. Tierney, Kevin McSorley, Conn L. Hastings, Sally-Ann Cryan, Timothy O'Brien, Mary J. Murphy, Frank P. Barry, Fergal J. O'Brien, Garry P. Duffy

Abstract

Gene therapy can be combined with tissue engineering constructs to produce gene-activated matrices (GAMs) with enhanced capacity for repair. Polyethyleneimine (PEI), a non-viral vector, has previously been optimised for high efficiency gene transfer in rat mesenchymal stem cells (rMSCs). The use of PEI to transfect human MSCs (hMSCs) with ephrinB2 is assessed here. Recently a role for the ephrinB2 ligand and EphB4 receptor duo has been proposed in bone remodelling. Herein, over-expression of the ephrinB2 ligand resulted in increased osteogenic differentiation in hMSCs. As ephrinB2 is a cell surface anchored ligand which only interacts with cells expressing the cognate EphB4 receptor through direct contact, we have shown that direct cell-cell contact between two neighbouring cells is responsible for enhanced osteogenesis. In an effort to begin to elucidate the molecular mechanisms at play downstream of ephrinB2 over-expression, RT-PCR was performed on the GAMs which revealed no significant changes in runx2 or BMP2 expression but an upregulation of osterix (Osx) and Dlx5 expression prompting the belief that the mode of osteogenesis is independent of the BMP2 pathway. This select interaction, coupled with the transient gene expression profile of PEI, makes the PEI-ephrinB2 GAM an ideal candidate matrix for a bone targeted GAM.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 2 6%
Poland 1 3%
Ireland 1 3%
Unknown 32 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 39%
Student > Master 5 14%
Researcher 5 14%
Professor 2 6%
Student > Postgraduate 2 6%
Other 5 14%
Unknown 3 8%
Readers by discipline Count As %
Engineering 8 22%
Agricultural and Biological Sciences 8 22%
Medicine and Dentistry 7 19%
Materials Science 2 6%
Social Sciences 1 3%
Other 3 8%
Unknown 7 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2012.
All research outputs
#9,290,902
of 12,096,642 outputs
Outputs from Journal of Controlled Release
#4,553
of 6,047 outputs
Outputs of similar age
#189,056
of 287,448 outputs
Outputs of similar age from Journal of Controlled Release
#37
of 62 outputs
Altmetric has tracked 12,096,642 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,047 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 287,448 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 62 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.