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Mendeley readers
Attention Score in Context
| Title |
Constitutional and somatic methylation status of DMRH19 and KvDMR in Wilms tumor patients
|
|---|---|
| Published in |
Genetics and Molecular Biology, January 2012
|
| DOI | 10.1590/s1415-47572012005000073 |
| Pubmed ID | |
| Authors |
Leila C.A. Cardoso, Jair A. Tenorio Castaño, Hanna S. Pereira, Maria Angélica de F.D. Lima, Anna Cláudia E. dos Santos, Paulo S. de Faria, Sima Ferman, Héctor N. Seuánez, Julián B. Nevado, José Carlos Cabral de Almeida, Pablo Lapunzina, Fernando R. Vargas |
| Abstract |
The most frequent epigenetic alterations in Wilms tumor (WT) occur at WT2, assigned to 11p15. WT2 consists of two domains: telomeric domain 1 (DMRH19) that contains the IGF2 gene and an imprinted maternally expressed transcript (H19) and centromeric domain 2 (KvDMR) that contains the genes KCNQ1, KCNQ1OT1 and CDKN1C. In this work, we used pyrosequencing and MS-MLPA to compare the methylation patterns of DMRH19/KvDMR in blood and tumor samples from 40 WT patients. Normal constitutional KvDMR methylation indicated that most of the epigenetic alterations in WT occur at DMRH19. Constitutional DMRH19 hypermethylation (HM DMRH19) was observed in two patients with Beckwith-Wiedemann syndrome. Pyrosequencing and MS-MLPA showed HM DMRH19 in 28/34 tumor samples: 16/34 with isolated HM DMRH19 and 12/34 with concomitant HM DMRH19 and KvDMR hypomethylation, indicating paternal uniparental disomy. With the exception of one blood sample, the MS-MLPA and pyrosequencing findings were concordant. Diffuse or focal anaplasia was present in five tumor samples and was associated with isolated somatic HM DMRH19 in four of them. Constitutional 11p15 methylation abnormalities were present in 5% of the samples and somatic abnormalities in the majority of tumors. Combined analysis of DMRH19/KvDMR by pyrosequencing and MS-MLPA is beneficial for characterizing epigenetic anomalies in WT, and MS-MLPA is useful and reliable for estimation of DNA methylation in a clinical setting. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 4% |
| Unknown | 24 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 16% |
| Professor | 3 | 12% |
| Researcher | 3 | 12% |
| Professor > Associate Professor | 3 | 12% |
| Student > Bachelor | 2 | 8% |
| Other | 4 | 16% |
| Unknown | 6 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 4 | 16% |
| Agricultural and Biological Sciences | 3 | 12% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Neuroscience | 2 | 8% |
| Business, Management and Accounting | 1 | 4% |
| Other | 4 | 16% |
| Unknown | 9 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 December 2012.
All research outputs
#20,656,161
of 25,374,647 outputs
Outputs from Genetics and Molecular Biology
#551
of 771 outputs
Outputs of similar age
#203,877
of 250,101 outputs
Outputs of similar age from Genetics and Molecular Biology
#7
of 12 outputs
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So far Altmetric has tracked 771 research outputs from this source. They receive a mean Attention Score of 4.8. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
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We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.