Title |
Human blood RNA stabilization in samples collected and transported for a large biobank
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Published in |
BMC Research Notes, September 2012
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DOI | 10.1186/1756-0500-5-510 |
Pubmed ID | |
Authors |
Nur Duale, Gunnar Brunborg, Kjersti S Rønningen, Thomas Briese, Jeanette Aarem, Kaja K Aas, Per Magnus, Camilla Stoltenberg, Ezra Susser, W Ian Lipkin |
Abstract |
The Norwegian Mother and Child Cohort Study (MoBa) is a nation-wide population-based pregnancy cohort initiated in 1999, comprising more than 108.000 pregnancies recruited between 1999 and 2008. In this study we evaluated the feasibility of integrating RNA analyses into existing MoBa protocols. We compared two different blood RNA collection tube systems - the PAXgene™ Blood RNA system and the Tempus™ Blood RNA system - and assessed the effects of suboptimal blood volumes in collection tubes and of transportation of blood samples by standard mail. Endpoints to characterize the samples were RNA quality and yield, and the RNA transcript stability of selected genes. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
France | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Denmark | 3 | 4% |
United Kingdom | 2 | 2% |
Netherlands | 1 | 1% |
United States | 1 | 1% |
Unknown | 78 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 25 | 29% |
Student > Ph. D. Student | 19 | 22% |
Student > Bachelor | 7 | 8% |
Student > Master | 5 | 6% |
Other | 5 | 6% |
Other | 14 | 16% |
Unknown | 10 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 20 | 24% |
Agricultural and Biological Sciences | 17 | 20% |
Biochemistry, Genetics and Molecular Biology | 15 | 18% |
Neuroscience | 3 | 4% |
Psychology | 3 | 4% |
Other | 13 | 15% |
Unknown | 14 | 16% |