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Metabolic determinants of the immune modulatory function of neural stem cells

Overview of attention for article published in Journal of Neuroinflammation, January 2016
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (58th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (54th percentile)

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5 tweeters
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2 Facebook pages

Citations

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9 Dimensions

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29 Mendeley
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Title
Metabolic determinants of the immune modulatory function of neural stem cells
Published in
Journal of Neuroinflammation, January 2016
DOI 10.1186/s12974-016-0667-7
Pubmed ID
Authors

Drago, Denise, Basso, Veronica, Gaude, Edoardo, Volpe, Giulio, Peruzzotti-Jametti, Luca, Bachi, Angela, Musco, Giovanna, Andolfo, Annapaola, Frezza, Christian, Mondino, Anna, Pluchino, Stefano, Denise Drago, Veronica Basso, Edoardo Gaude, Giulio Volpe, Luca Peruzzotti-Jametti, Angela Bachi, Giovanna Musco, Annapaola Andolfo, Christian Frezza, Anna Mondino, Stefano Pluchino

Abstract

Neural stem cells (NSCs) display tissue trophic and immune modulatory therapeutic activities after transplantation in central nervous system disorders. The intercellular interplay between stem cells and target immune cells is increased in NSCs exposed to inflammatory cues. Here, we hypothesize that inflammatory cytokine signalling leads to metabolic reprogramming of NSCs regulating some of their immune modulatory effects. NSC lines were prepared from the subventricular zone (SVZ) of 7-12-week-old mice. Whole secretome-based screening and analysis of intracellular small metabolites was performed in NSCs exposed to cocktails of either Th1-like (IFN-γ, 500 U/ml; TNF-α, 200 U/ml; IL-1β, 100 U/ml) or Th2-like (IL-4, IL-5 and IL-13; 10 ng/ml) inflammatory cytokines for 16 h in vitro. Isotopologues distribution of arginine and downstream metabolites was assessed by liquid chromatography/mass spectrometry in NSCs incubated with U-(13)C6 L-arginine in the presence or absence of Th1 or Th2 cocktails (Th1 NSCs or Th2 NSCs). The expression of arginase I and II was investigated in vitro in Th1 NSCs and Th2 NSCs and in vivo in the SVZ of mice with experimental autoimmune encephalomyelitis, as prototypical model of Th1 cell-driven brain inflammatory disease. The effects of the inflammatory cytokine signalling were studied in NSC-lymph node cells (LNC) co-cultures by flow cytometry-based analysis of cell proliferation following pan-arginase inhibition with N(ω)-hydroxy-nor-arginine (nor-NOHA). Cytokine-primed NSCs showed significantly higher anti-proliferative effect in co-cultures vs. control NSCs. Metabolomic analysis of intracellular metabolites revealed alteration of arginine metabolism and increased extracellular arginase I activity in cytokine-primed NSCs. Arginase inhibition by nor-NOHA partly rescued the anti-proliferative effects of cytokine-primed NSCs. Our work underlines the use of metabolic profiling as hypothesis-generating tools that helps unravelling how stem cell-mediated mechanisms of tissue restoration become affected by local inflammatory responses. Among different therapeutic candidates, we identify arginase signalling as novel metabolic determinant of the NSC-to-immune system communication.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 31%
Researcher 7 24%
Student > Bachelor 6 21%
Other 3 10%
Unspecified 2 7%
Other 2 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 21%
Neuroscience 5 17%
Biochemistry, Genetics and Molecular Biology 5 17%
Unspecified 4 14%
Medicine and Dentistry 4 14%
Other 5 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 July 2018.
All research outputs
#7,024,490
of 13,266,991 outputs
Outputs from Journal of Neuroinflammation
#607
of 1,548 outputs
Outputs of similar age
#104,894
of 262,126 outputs
Outputs of similar age from Journal of Neuroinflammation
#21
of 48 outputs
Altmetric has tracked 13,266,991 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,548 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,126 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.
We're also able to compare this research output to 48 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.