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Development of butanol-tolerant Bacillus subtilis strain GRSW2-B1 as a potential bioproduction host

Overview of attention for article published in AMB Express, May 2011
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Title
Development of butanol-tolerant Bacillus subtilis strain GRSW2-B1 as a potential bioproduction host
Published in
AMB Express, May 2011
DOI 10.1186/2191-0855-1-10
Pubmed ID
Abstract

As alternative microbial hosts for butanol production with organic-solvent tolerant trait are in high demands, a butanol-tolerant bacterium, Bacillus subtilis GRSW2-B1, was thus isolated. Its tolerance covered a range of organic solvents at high concentration (5%v/v), with remarkable tolerance in particular to butanol and alcohol groups. It was susceptible for butanol acclimatization, which resulted in significant tolerance improvement. It has versatility for application in a variety of fermentation process because it has superior tolerance when cells were exposed to butanol either as high-density, late-exponential grown cells (up to 5%v/v) or under growing conditions (up to 2.25%v/v). Genetic transformation procedure was optimized, yielding the highest efficiency at 5.17 × 103 colony forming unit (μg DNA)-1. Gene expression could be effectively driven by several promoters with different levels, where as the highest expression was observed with a xylose promoter. The constructed vector was stably maintained in the transformants, in the presence or absence of butanol stress. Adverse effect of efflux-mediated tetracycline resistance determinant (TetL) to bacterial organic-solvent tolerance property was unexpectedly observed and thus discussed. Overall results indicate that B. subtilis GRSW2-B1 has potential to be engineered and further established as a genetic host for bioproduction of butanol.

Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Malaysia 2 3%
Brazil 1 2%
Colombia 1 2%
India 1 2%
Costa Rica 1 2%
New Zealand 1 2%
Unknown 51 88%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 33%
Researcher 14 24%
Student > Master 7 12%
Professor > Associate Professor 4 7%
Student > Doctoral Student 3 5%
Other 8 14%
Unknown 3 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 57%
Biochemistry, Genetics and Molecular Biology 11 19%
Engineering 5 9%
Immunology and Microbiology 1 2%
Medicine and Dentistry 1 2%
Other 2 3%
Unknown 5 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 November 2011.
All research outputs
#2,901,284
of 3,629,283 outputs
Outputs from AMB Express
#57
of 148 outputs
Outputs of similar age
#224,143
of 280,637 outputs
Outputs of similar age from AMB Express
#2
of 13 outputs
Altmetric has tracked 3,629,283 research outputs across all sources so far. This one is in the 2nd percentile – i.e., 2% of other outputs scored the same or lower than it.
So far Altmetric has tracked 148 research outputs from this source. They receive a mean Attention Score of 1.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,637 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.