Chapter title |
An Effective and Reliable Xeno-free Cryopreservation Protocol for Single Human Pluripotent Stem Cells
|
---|---|
Chapter number | 322 |
Book title |
Stem Cell Heterogeneity
|
Published in |
Methods in molecular biology, April 2016
|
DOI | 10.1007/7651_2016_322 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6549-6, 978-1-4939-6550-2
|
Authors |
Guoliang Meng, Anna Poon, Shiying Liu, Derrick E. Rancourt, Meng, Guoliang, Poon, Anna, Liu, Shiying, Rancourt, Derrick E. |
Abstract |
Efficient cryopreservation of human pluripotent stem cells (hPSCs) in chemically defined, xeno-free conditions is highly desirable for medical research and clinical applications such as cell-based therapies. Here we present a simple and effective slow freezing-rapid thawing protocol for the cryopreservation of feeder-free, single hPSCs. This cryopreservation protocol involves the supplementation of 10 % dimethyl sulfoxide (DMSO) and 10 μM Rho-associated kinase inhibitor Y-27632 into two types of xeno-free, defined media supplements (Knockout Serum Replacement and TeSR2). High post-thaw cell recovery (~90 %) and cell expansion (~70 %) can be achieved using this protocol. The cryopreserved single cells retain the morphological characteristics of hPSCs and differentiation capabilities of pluripotent stem cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 33% |
Other | 2 | 22% |
Student > Bachelor | 1 | 11% |
Unknown | 3 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Engineering | 2 | 22% |
Immunology and Microbiology | 1 | 11% |
Sports and Recreations | 1 | 11% |
Unknown | 3 | 33% |