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Mendeley readers
| Chapter title |
Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro
|
|---|---|
| Chapter number | 320 |
| Book title |
Stem Cell Heterogeneity
|
| Published in |
Methods in molecular biology, April 2016
|
| DOI | 10.1007/7651_2016_320 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6549-6, 978-1-4939-6550-2
|
| Authors |
Yukiteru Ouji, Masahide Yoshikawa, Ouji, Yukiteru, Yoshikawa, Masahide |
| Abstract |
CD49f+ CD34+ cells, a population rich in skin epithelial stem cells (EpSCs), were obtained from adult mouse skin and cultured with Wnt-3a for 10 days. On day 10, CD49f+ CD34+ cells were sorted and subjected to a second 10-day culture with Wnt-3a. The same procedures were repeated until fifteenth 10-day culture. CD49f+ CD34+ cells obtained from each 10-day culture retained the same EpSC-characteristics as seen in the original EpSCs from adult mouse skin. Here, wedescribe the culture protocol using Wnt-3a for successful maintenance of EpSCs. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 1 | 17% |
| Unknown | 5 | 83% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 1 | 17% |
| Unknown | 5 | 83% |