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Chapter title |
Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro
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Chapter number | 328 |
Book title |
Stem Cell Heterogeneity
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Published in |
Methods in molecular biology, April 2016
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DOI | 10.1007/7651_2016_328 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6549-6, 978-1-4939-6550-2
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Authors |
Masahide Yoshikawa, Yukiteru Ouji, Yoshikawa, Masahide, Ouji, Yukiteru |
Abstract |
Inner ear hair cells (HCs) function as the primary transducers for perception of sound and balance, while a defect in their formation or their loss results in sensory deficits. In mammals, once HCs are lost, they are not regenerated, and thus, various medical strategies have been proposed for their reproduction. Although embryonic stem (ES) cells are a promising source for cell therapy, little is known about efficient generation of hair cell-like cells (HCLs) from mouse ES cells. In the present protocol, we describe a simple method for obtaining ES-derived murine HCLs (HIST2 method). |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 2 | 50% |
Student > Ph. D. Student | 1 | 25% |
Student > Master | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 50% |
Agricultural and Biological Sciences | 1 | 25% |
Engineering | 1 | 25% |