↓ Skip to main content

Stem Cell Heterogeneity

Overview of attention for book
Cover of 'Stem Cell Heterogeneity'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 315 Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice
  3. Altmetric Badge
    Chapter 319 Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro
  4. Altmetric Badge
    Chapter 320 Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro
  5. Altmetric Badge
    Chapter 321 Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies
  6. Altmetric Badge
    Chapter 322 An Effective and Reliable Xeno-free Cryopreservation Protocol for Single Human Pluripotent Stem Cells
  7. Altmetric Badge
    Chapter 323 Induction of a Tumor-Metastasis-Receptive Microenvironment as an Unwanted Side Effect After Radio/Chemotherapy and In Vitro and In Vivo Assays to Study this Phenomenon
  8. Altmetric Badge
    Chapter 324 Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing
  9. Altmetric Badge
    Chapter 325 Stencil Micropatterning for Spatial Control of Human Pluripotent Stem Cell Fate Heterogeneity
  10. Altmetric Badge
    Chapter 326 Isolation and Characterization of Cancer Stem Cells of the Non-Small-Cell Lung Cancer (A549) Cell Line
  11. Altmetric Badge
    Chapter 327 Erratum to: Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method
  12. Altmetric Badge
    Chapter 327 Aerosol-Based Cell Therapy for Treatment of Lung Diseases
  13. Altmetric Badge
    Chapter 328 Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro
  14. Altmetric Badge
    Chapter 329 In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry
  15. Altmetric Badge
    Chapter 342 Isolation and Propagation of Glioma Stem Cells from Acutely Resected Tumors
  16. Altmetric Badge
    Chapter 343 Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter
  17. Altmetric Badge
    Chapter 344 Automated Cell-Based Quantitation of 8-OHdG Damage
  18. Altmetric Badge
    Chapter 345 Heterogeneity of Stem Cells: A Brief Overview
  19. Altmetric Badge
    Chapter 346 Agent-Based Modeling of Cancer Stem Cell Driven Solid Tumor Growth.
  20. Altmetric Badge
    Chapter 347 Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells.
  21. Altmetric Badge
    Chapter 348 Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method
  22. Altmetric Badge
    Chapter 349 Visualizing the Functional Heterogeneity of Muscle Stem Cells
  23. Altmetric Badge
    Chapter 350 CoCl2 Administration to Vascular MSC Cultures as an In Vitro Hypoxic System to Study Stem Cell Survival and Angiogenesis
  24. Altmetric Badge
    Chapter 356 Dissecting Transcriptional Heterogeneity in Pluripotency: Single Cell Analysis of Mouse Embryonic Stem Cells
  25. Altmetric Badge
    Chapter 357 Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs)
  26. Altmetric Badge
    Chapter 358 Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells.
  27. Altmetric Badge
    Chapter 360 Reporter Systems to Study Cancer Stem Cells
  28. Altmetric Badge
    Chapter 361 Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells
  29. Altmetric Badge
    Chapter 362 Growth Factor-Free Pre-vascularization of Cell Sheets for Tissue Engineering
Attention for Chapter 358: Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells.
Altmetric Badge

Mentioned by

twitter
1 tweeter

Citations

dimensions_citation
1 Dimensions

Readers on

mendeley
9 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells.
Chapter number 358
Book title
Stem Cell Heterogeneity
Published in
Methods in molecular biology, May 2016
DOI 10.1007/7651_2016_358
Pubmed ID
Book ISBNs
978-1-4939-6549-6, 978-1-4939-6550-2
Authors

Krzysztof Szade, Monika Zukowska, Alicja Jozkowicz, Jozef Dulak

Abstract

The metabolism of quiescent adult stem cells differs from the metabolism of differentiated cells. The metabolic processes are tightly regulated and their alterations disturb function of stem cells. One of the indicators of metabolic status of cells is the ATP level. While the method of measuring the ATP levels has been known for many years, estimating ATP levels in small population of defined stem cells isolated directly from the tissue has remained challenging. Here, we show our method of measuring the ATP levels in hematopoietic stem cells sorted from murine bone marrow. We used magnetic sorting as well as cell sorter and adopted the commonly used bioluminescence-based detection kits in described protocol. Our strategy allows to measure ATP levels in 1000 highly purified HSC.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 22%
Student > Bachelor 1 11%
Professor 1 11%
Other 1 11%
Student > Master 1 11%
Other 2 22%
Unknown 1 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 22%
Agricultural and Biological Sciences 2 22%
Nursing and Health Professions 1 11%
Medicine and Dentistry 1 11%
Chemistry 1 11%
Other 1 11%
Unknown 1 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 May 2016.
All research outputs
#6,655,782
of 7,693,476 outputs
Outputs from Methods in molecular biology
#3,861
of 5,462 outputs
Outputs of similar age
#224,809
of 269,122 outputs
Outputs of similar age from Methods in molecular biology
#11
of 17 outputs
Altmetric has tracked 7,693,476 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,462 research outputs from this source. They receive a mean Attention Score of 1.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 269,122 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.