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Prokineticin 2 antagonist, PKRA7 suppresses arthritis in mice with collagen-induced arthritis

Overview of attention for article published in BMC Musculoskeletal Disorders, September 2016
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Title
Prokineticin 2 antagonist, PKRA7 suppresses arthritis in mice with collagen-induced arthritis
Published in
BMC Musculoskeletal Disorders, September 2016
DOI 10.1186/s12891-016-1243-0
Pubmed ID
Authors

Haruyasu Ito, Kentaro Noda, Ken Yoshida, Kazuhiro Otani, Masayuki Yoshiga, Yohsuke Oto, Saburo Saito, Daitaro Kurosaka

Abstract

Prokineticin 2 (PK2) expression is upregulated in mice with collagen-induced arthritis (CIA), an animal model of rheumatoid arthritis. The purpose of our study was to investigate the effects of PK2 inhibition on CIA. PK2, prokineticin receptor (PKR) 1, and PKR2 mRNA transcripts in the joints of CIA mice were measured by real-time PCR on Days 21, 28, and 35 (n = 15/day). Localization of PKR1 and PKR2 proteins was examined immunohistochemically. PKRA7, a PK2 antagonist, was administered intraperitoneally for 2 weeks to CIA mice, and the severity of arthritis was compared between treated (n = 12) and untreated (n = 12) mice. The gene expression levels of inflammatory cytokines IL-1β, IL-6, TNF-α, and VEGF were also measured by real-time PCR and compared between treated (n = 6) and untreated (n = 6) CIA mice. The data was statistically analyzed, and P values of less than 0.05 were considered significant. In the thickened synovial membrane, PKR1 protein was expressed in infiltrating neutrophils, while PKR2 expression was found in macrophage-like mononuclear cells. PK2 gene expression was significantly more pronounced on Days 28 and 35 than on Day 21 (2.15 and 2.03 versus 1.00, P = 0.0311 and 0.0247; Dunn's multiple comparison). PKR2 gene expression levels were significantly higher on Days 28 and 35 compared to Day 21 (25.4 and 39.3 versus 1.0, P = 0.002 and < 0.0001; Dunn's multiple comparison). Administration of PKRA7 suppressed the severity of arthritis (P < 0.001; two-way analysis of variance). A gene expression analysis of inflammatory cytokines revealed significantly reduced IL-1β and lL-6 expression in the joints of PKRA7-treated mice compared to untreated mice (0.1 versus 1.0, P = 0.0043 and 0.04 versus 1.0, P = 0.0022, respectively; Mann-Whitney test). PK2 inhibition suppressed arthritis in mice with CIA.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 17%
Other 2 17%
Student > Doctoral Student 2 17%
Student > Bachelor 1 8%
Student > Ph. D. Student 1 8%
Other 3 25%
Unknown 1 8%
Readers by discipline Count As %
Medicine and Dentistry 10 83%
Biochemistry, Genetics and Molecular Biology 1 8%
Unknown 1 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 September 2016.
All research outputs
#6,340,151
of 8,355,069 outputs
Outputs from BMC Musculoskeletal Disorders
#1,676
of 2,110 outputs
Outputs of similar age
#178,347
of 252,739 outputs
Outputs of similar age from BMC Musculoskeletal Disorders
#48
of 66 outputs
Altmetric has tracked 8,355,069 research outputs across all sources so far. This one is in the 13th percentile – i.e., 13% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,110 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 252,739 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 66 others from the same source and published within six weeks on either side of this one. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.