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Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles

Overview of attention for article published in Journal of Molecular Medicine, September 2016
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  • High Attention Score compared to outputs of the same age and source (90th percentile)

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2 tweeters
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1 patent

Citations

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19 Dimensions

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48 Mendeley
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Title
Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles
Published in
Journal of Molecular Medicine, September 2016
DOI 10.1007/s00109-016-1471-7
Pubmed ID
Authors

Sylwia Bobis-Wozowicz, Katarzyna Kmiotek, Karolina Kania, Elzbieta Karnas, Anna Labedz-Maslowska, Malgorzata Sekula, Sylwia Kedracka-Krok, Jacek Kolcz, Dariusz Boruczkowski, Zbigniew Madeja, Ewa K. Zuba-Surma

Abstract

Growing evidence indicates that intracellular signaling mediated by extracellular vesicles (EVs) released by stem cells plays a considerable role in triggering the regenerative program upon transplantation. EVs from umbilical cord mesenchymal stem cells (UC-MSC-EVs) have been shown to enhance tissue repair in animal models. However, translating such results into clinical practice requires optimized EV collection procedures devoid of animal-originating agents. Thus, in this study, we analyzed the influence of xeno-free expansion media on biological properties of UC-MSCs and UC-MSC-EVs for future applications in cardiac repair in humans. Our results show that proliferation, differentiation, phenotype stability, and cytokine secretion by UC-MSCs vary depending on the type of xeno-free media. Importantly, we found distinct molecular and functional properties of xeno-free UC-MSC-EVs including enhanced cardiomyogenic and angiogenic potential impacting on target cells, which may be explained by elevated concentration of several pro-cardiogenic and pro-angiogenic microRNA (miRNAs) present in the EVs. Our data also suggest predominantly low immunogenic capacity of certain xeno-free UC-MSC-EVs reflected by their inhibitory effect on proliferation of immune cells in vitro. Summarizing, conscious selection of cell culture conditions is required to harvest UC-MSC-EVs with the optimal desired properties including enhanced cardiac and angiogenic capacity, suitable for tissue regeneration. Type of xeno-free media influences biological properties of UC-MSCs in vitro. Certain xeno-free media promote proliferation and differentiation ability of UC-MSCs. EVs collected from xeno-free cultures of UC-MSCs are biologically active. Xeno-free UC-MSC-EVs enhance cardiac and angiogenic potential of target cells. Type of xeno-free media determines immunomodulatory effects mediated by UC-MSC-EVs.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Poland 1 2%
Unknown 47 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 31%
Researcher 9 19%
Student > Doctoral Student 5 10%
Student > Bachelor 5 10%
Student > Master 4 8%
Other 7 15%
Unknown 3 6%
Readers by discipline Count As %
Medicine and Dentistry 12 25%
Biochemistry, Genetics and Molecular Biology 10 21%
Engineering 7 15%
Agricultural and Biological Sciences 6 13%
Immunology and Microbiology 3 6%
Other 6 13%
Unknown 4 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2020.
All research outputs
#4,233,386
of 14,941,954 outputs
Outputs from Journal of Molecular Medicine
#308
of 1,225 outputs
Outputs of similar age
#84,661
of 267,957 outputs
Outputs of similar age from Journal of Molecular Medicine
#2
of 20 outputs
Altmetric has tracked 14,941,954 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 1,225 research outputs from this source. They receive a mean Attention Score of 4.4. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,957 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.