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Functional Organization of Repeat Addition Processivity and DNA Synthesis Determinants in the Human Telomerase Multimer

Overview of attention for article published in Molecular & Cellular Biology, May 2004
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Title
Functional Organization of Repeat Addition Processivity and DNA Synthesis Determinants in the Human Telomerase Multimer
Published in
Molecular & Cellular Biology, May 2004
DOI 10.1128/mcb.24.9.3720-3733.2004
Pubmed ID
Authors

Tara J. Moriarty, Delphine T. Marie-Egyptienne, Chantal Autexier

Abstract

Human telomerase is a multimer containing two human telomerase RNAs (hTRs) and most likely two human telomerase reverse transcriptases (hTERTs). Telomerase synthesizes multiple telomeric repeats using a unique repeat addition form of processivity. We investigated hTR and hTERT sequences that were essential for DNA synthesis and processivity using a direct primer extension telomerase assay. We found that hTERT consists of two physically separable functional domains, a polymerase domain containing RNA interaction domain 2 (RID2), reverse transcriptase (RT), and C-terminal sequences, and a major accessory domain, RNA interaction domain 1 (RID1). RID2 mutants defective in high-affinity hTR interactions and an RT catalytic mutant exhibited comparable DNA synthesis defects. The RID2-interacting hTR P6.1 helix was also essential for DNA synthesis. RID1 interacted with the hTR pseudoknot-template domain and hTERT's RT motifs and putative thumb and was essential for processivity, but not DNA synthesis. The hTR pseudoknot was essential for processivity, but not DNA synthesis, and processivity was reduced or abolished in dimerization-defective pseudoknot mutants. trans-acting hTERTs and hTRs complemented the processivity defects of RID1 and pseudoknot mutants, respectively. These data provide novel insight into the catalytic organization of the human telomerase complex and suggest that repeat addition processivity is one of the major catalytic properties conferred by telomerase multimerization.

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Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 2 6%
United States 2 6%
United Kingdom 1 3%
Unknown 27 84%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 56%
Researcher 6 19%
Professor 2 6%
Student > Bachelor 2 6%
Student > Master 2 6%
Other 2 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 21 66%
Biochemistry, Genetics and Molecular Biology 4 13%
Medicine and Dentistry 4 13%
Immunology and Microbiology 1 3%
Social Sciences 1 3%
Other 1 3%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 September 2016.
All research outputs
#6,412,368
of 7,408,468 outputs
Outputs from Molecular & Cellular Biology
#3,492
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Outputs of similar age
#200,277
of 240,159 outputs
Outputs of similar age from Molecular & Cellular Biology
#34
of 39 outputs
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