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The C terminus of the human telomerase reverse transcriptase is a determinant of enzyme processivity

Overview of attention for article published in Nucleic Acids Research, July 2003
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Title
The C terminus of the human telomerase reverse transcriptase is a determinant of enzyme processivity
Published in
Nucleic Acids Research, July 2003
DOI 10.1093/nar/gkg437
Pubmed ID
Authors

Sylvain Huard, Tara J. Moriarty, Chantal Autexier

Abstract

The catalytic subunit of telomerase (TERT) contains conserved reverse transcriptase-like motifs but N- and C-terminal regions unique to telomerases. Despite weak sequence conservation, the C terminus of TERTs from various organisms has been implicated in telomerase-specific functions, including telomerase activity, functional multimerization with other TERT molecules, enzyme processivity and telomere length maintenance. We studied hTERT proteins containing small C-terminal deletions or substitutions to identify and characterize hTERT domains mediating telomerase activity, hTERT multimerization and processivity. Using sequence alignment of five vertebrate TERTs and Arabidopsis thaliana TERT, we identified blocks of highly conserved amino acids that were required for human telomerase activity and functional hTERT complementation. We adapted the non-PCR-based telomerase elongation assay to characterize telomerase expressed and reconstituted in the in vitro transcription/translation rabbit reticulocyte lysate system. Using this assay, we found that the hTERT C terminus, like the C terminus of Saccharomyces cerevisiae TERT, contributes to successive nucleotide addition within a single 6-base telomeric repeat (type I processivity). Certain mutations in the hTERT C terminus also reduced the repetitive addition of multiple telomeric repeats (type II processivity). Our results suggest a functionally conserved role for the TERT C terminus in telomerase enzyme processivity.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 2 5%
United States 2 5%
Germany 1 3%
United Kingdom 1 3%
Turkey 1 3%
Canada 1 3%
Unknown 32 80%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 43%
Researcher 8 20%
Student > Bachelor 4 10%
Student > Master 4 10%
Professor 3 8%
Other 4 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 78%
Biochemistry, Genetics and Molecular Biology 4 10%
Medicine and Dentistry 3 8%
Immunology and Microbiology 1 3%
Chemistry 1 3%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 September 2016.
All research outputs
#6,377,721
of 8,403,724 outputs
Outputs from Nucleic Acids Research
#8,995
of 10,918 outputs
Outputs of similar age
#178,796
of 254,070 outputs
Outputs of similar age from Nucleic Acids Research
#229
of 255 outputs
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We're also able to compare this research output to 255 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.