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Functional Multimerization of Human Telomerase Requires an RNA Interaction Domain in the N Terminus of the Catalytic Subunit

Overview of attention for article published in Molecular & Cellular Biology, February 2002
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Title
Functional Multimerization of Human Telomerase Requires an RNA Interaction Domain in the N Terminus of the Catalytic Subunit
Published in
Molecular & Cellular Biology, February 2002
DOI 10.1128/mcb.22.4.1253-1265.2002
Pubmed ID
Authors

Tara J. Moriarty, Sylvain Huard, Sophie Dupuis, Chantal Autexier

Abstract

Functional human telomerase complexes are minimally composed of the human telomerase RNA (hTR) and a catalytic subunit (human telomerase reverse transcriptase [hTERT]) containing reverse transcriptase (RT)-like motifs. The N terminus of TERT proteins is unique to the telomerase family and has been implicated in catalysis, telomerase RNA binding, and telomerase multimerization, and conserved motifs have been identified by alignment of TERT sequences from multiple organisms. We studied hTERT proteins containing N-terminal deletions or substitutions to identify and characterize hTERT domains mediating telomerase catalytic activity, hTR binding, and hTERT multimerization. Using multiple sequence alignment, we identified two vertebrate-conserved TERT N-terminal regions containing vertebrate-specific residues that were required for human telomerase activity. We identified two RNA interaction domains, RID1 and RID2, the latter containing a vertebrate-specific RNA binding motif. Mutations in RID2 reduced the association of hTR with hTERT by 50 to 70%. Inactive mutants defective in RID2-mediated hTR binding failed to complement an inactive hTERT mutant containing an RT motif substitution to reconstitute activity. Our results suggest that functional hTERT complementation requires intact RID2 and RT domains on the same hTERT molecule and is dependent on hTR and the N terminus.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 2 5%
United States 2 5%
India 1 3%
Serbia 1 3%
Canada 1 3%
Unknown 30 81%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 20 54%
Researcher 7 19%
Professor 3 8%
Student > Master 3 8%
Student > Doctoral Student 2 5%
Other 2 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 59%
Biochemistry, Genetics and Molecular Biology 8 22%
Medicine and Dentistry 3 8%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Immunology and Microbiology 1 3%
Other 2 5%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 September 2016.
All research outputs
#6,377,721
of 8,403,724 outputs
Outputs from Molecular & Cellular Biology
#3,471
of 3,952 outputs
Outputs of similar age
#178,796
of 254,070 outputs
Outputs of similar age from Molecular & Cellular Biology
#29
of 39 outputs
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