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Cell Migration

Overview of attention for book
Cover of 'Cell Migration'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Cell migration: an overview.
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    Chapter 2 Scratch-wound assay.
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    Chapter 3 HGF-induced DU145 cell scatter assay.
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    Chapter 4 Using the Dunn chemotaxis chamber to analyze primary cell migration in real time.
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    Chapter 5 Imaging cells within 3D cell-derived matrix.
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    Chapter 6 Live cell imaging of neuronal growth cone motility and guidance in vitro.
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    Chapter 7 Boyden chamber.
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    Chapter 8 Transwell(®) invasion assays.
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    Chapter 9 Imaging podosome dynamics and matrix degradation.
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    Chapter 10 Endothelial cell migration under flow.
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    Chapter 11 In vitro analysis of chemotactic leukocyte migration in 3D environments.
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    Chapter 12 Quantification of transendothelial migration using three-dimensional confocal microscopy.
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    Chapter 13 Chemotaxis of slow migrating Mammalian cells analysed by video microscopy.
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    Chapter 14 Live cell fluorescence microscopy techniques.
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    Chapter 15 Measuring invasion in an organotypic model.
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    Chapter 16 Analysis of cell migration using Caenorhabditis elegans as a model system.
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    Chapter 17 Drosophila hemocyte migration: an in vivo assay for directional cell migration.
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    Chapter 18 Measuring inflammatory cell migration in the zebrafish.
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    Chapter 19 Border cell migration: a model system for live imaging and genetic analysis of collective cell movement.
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    Chapter 20 Assessment of development and chemotaxis in Dictyostelium discoideum mutants.
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    Chapter 21 Simple experimental and spontaneous metastasis assays in mice.
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    Chapter 22 Two-photon intravital multicolour imaging to study metastatic behaviour of cancer cells in vivo.
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    Chapter 23 Measuring angiogenesis in mice.
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    Chapter 24 Time-lapse imaging of chick cardiac precursor cells.
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    Chapter 25 Characterizing system performance in total internal reflection fluorescence microscopy.
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    Chapter 26 Fluorescence recovery after photobleaching.
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    Chapter 27 Measuring FRET using time-resolved FLIM.
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    Chapter 28 Cell migration in confinement: a micro-channel-based assay.
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    Chapter 29 Functional screening with a live cell imaging-based random cell migration assay.
  31. Altmetric Badge
    Chapter 30 Beads on the run: beads as alternative tools for chemotaxis assays.
Attention for Chapter 13: Chemotaxis of slow migrating Mammalian cells analysed by video microscopy.
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  • Average Attention Score compared to outputs of the same age and source

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Chapter title
Chemotaxis of slow migrating Mammalian cells analysed by video microscopy.
Chapter number 13
Book title
Cell Migration
Published in
Methods in molecular biology, June 2011
DOI 10.1007/978-1-61779-207-6_13
Pubmed ID
Book ISBNs
978-1-61779-206-9, 978-1-61779-207-6
Authors

Zantl R, Horn E, Roman Zantl, Elias Horn, Zantl, Roman, Horn, Elias

Editors

Claire M. Wells, Maddy Parsons

Abstract

We present a microfabricated chamber designed for visualising and quantifying the chemotaxis of slow-migrating adherent mammalian cells such as cancer and endothelial cells. Most of the existing solutions for the investigation of chemotaxis are limited to fast migrating cells such as leukocytes or Dictyostelium discoideum. Here, we describe the details of an assay using the μ-Slide Chemotaxis to investigate the chemotactic response of human umbilical vein endothelial cells to a gradient of human vascular endothelial growth factor 165. In combination with phase contrast video microscopy and cell tracking, the trajectories of all single cells migrating in temporally stable gradients are derived. The resulting migration data are displayed and analysed in detail by several different parameters for quantifying chemotaxis. We found that with this tool the potential of chemoattractants to migration of mammalian cells as well as the impact of inhibitors to chemotaxis and migration can be evaluated.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 54%
Researcher 3 23%
Student > Master 2 15%
Unknown 1 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 46%
Physics and Astronomy 2 15%
Biochemistry, Genetics and Molecular Biology 1 8%
Medicine and Dentistry 1 8%
Neuroscience 1 8%
Other 0 0%
Unknown 2 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 October 2015.
All research outputs
#2,101,265
of 6,335,899 outputs
Outputs from Methods in molecular biology
#698
of 4,634 outputs
Outputs of similar age
#20,811
of 59,320 outputs
Outputs of similar age from Methods in molecular biology
#5
of 9 outputs
Altmetric has tracked 6,335,899 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 4,634 research outputs from this source. They receive a mean Attention Score of 1.5. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 59,320 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.