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Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1

Overview of attention for article published in Veterinary Research, September 2016
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Title
Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1
Published in
Veterinary Research, September 2016
DOI 10.1186/s13567-016-0378-1
Pubmed ID
Authors

Luisa Samaniego-Barrón, Sarahí Luna-Castro, Carolina Piña-Vázquez, Francisco Suárez-Güemes, Mireya de la Garza

Abstract

Mannheimia haemolytica is a Gram negative bacterium that is part of the bovine respiratory disease, which causes important economic losses in the livestock industry. In the present work, the interaction between M. haemolytica A1 and bovine lactoferrin (BLf) was studied. This iron-chelating glycoprotein is part of the mammalian innate-immune system and is present in milk and mucosal secretions; Lf is also contained in neutrophils secondary granules, which release this glycoprotein at infection sites. It was evidenced that M. haemolytica was not able to use iron-charged BLf (BholoLf) as a sole iron source; nevertheless, iron-lacked BLf (BapoLf) showed a bactericidal effect against M. haemolytica with MIC of 4.88 ± 1.88 and 7.31 ± 1.62 μM for M. haemolytica strain F (field isolate) and M. haemolytica strain R (reference strain), respectively. Through overlay assays and 2-D electrophoresis, two OMP of 32.9 and 34.2 kDa with estimated IP of 8.18 and 9.35, respectively, were observed to bind both BapoLf and BholoLf; these OMP were identified by Maldi-Tof as OmpA (heat-modifiable OMP) and a membrane protein (porin). These M. haemolytica BLf binding proteins could be interacting in vivo with both forms of BLf depending on the iron state of the bovine.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 3%
Unknown 35 97%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 22%
Student > Master 7 19%
Researcher 4 11%
Professor 2 6%
Student > Ph. D. Student 2 6%
Other 4 11%
Unknown 9 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 28%
Biochemistry, Genetics and Molecular Biology 5 14%
Veterinary Science and Veterinary Medicine 4 11%
Immunology and Microbiology 4 11%
Medicine and Dentistry 2 6%
Other 0 0%
Unknown 11 31%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 September 2016.
All research outputs
#6,388,671
of 8,418,826 outputs
Outputs from Veterinary Research
#438
of 604 outputs
Outputs of similar age
#178,406
of 253,723 outputs
Outputs of similar age from Veterinary Research
#19
of 23 outputs
Altmetric has tracked 8,418,826 research outputs across all sources so far. This one is in the 13th percentile – i.e., 13% of other outputs scored the same or lower than it.
So far Altmetric has tracked 604 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
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We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.