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Protein Blotting and Detection

Overview of attention for book
Cover of 'Protein Blotting and Detection'

Table of Contents

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    Book Overview
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    Chapter 1 Origins of protein blotting.
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    Chapter 2 Protein Blotting and Detection
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    Chapter 3 Introduction to protein blotting.
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    Chapter 4 Simultaneous immunoblotting analysis with activity gel electrophoresis and 2-D gel electrophoresis.
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    Chapter 5 Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support.
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    Chapter 6 Tryptic peptide purification using polyvinylidene difluoride membrane for matrix-assisted laser desorption ionization time of flight mass spectrometry.
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    Chapter 7 Affinity immunoblotting for analysis of antibody clonotype distribution in a lupus patient developing anti-Ro 60 over time.
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    Chapter 8 Nonelectrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple antigens to obtain 12 immunoblots.
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    Chapter 9 Slice blotting.
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    Chapter 10 Localizing proteins by tissue printing.
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    Chapter 11 Dot-immunobinding assay.
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    Chapter 12 Capillary blotting of glycosaminoglycans on nitrocellulose membranes after agarose-gel electrophoresis separation.
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    Chapter 13 Quantitative computerized western blotting.
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    Chapter 14 Cationic electrophoresis and eastern blotting.
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    Chapter 15 A miniaturized blotting system for simultaneous detecting of different autoantibodies.
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    Chapter 16 Transfer and multiplex immunoblotting of a paraffin embedded tissue.
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    Chapter 17 Multistrip western blotting to increase quantitative data output.
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    Chapter 18 Western Blotting/Edman Sequencing using PVDF Membrane
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    Chapter 19 Blotting from PhastGel to membranes by ultrasound.
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    Chapter 20 Ultrarapid electrophoretic transfer of high and low molecular weight proteins using heat.
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    Chapter 21 Strip immunoblotting of multiple antigenic peptides to nitrocellulose membrane.
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    Chapter 22 Affinity purification of autoantibodies from an antigen strip excised from a nitrocellulose protein blot.
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    Chapter 23 Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures.
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    Chapter 24 Efficient electroblotting of very large proteins using a vertical agarose electrophoresis system.
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    Chapter 25 Semi-dry protein transfer and immunodetection of P-selectin using an antibody to its C-terminal tag.
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    Chapter 26 The multiple antigen blot assay: a simple, versatile and multipurpose immunoenzymatic technique.
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    Chapter 27 Blotting from immobilized pH gradient gels: application to total cell lysates.
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    Chapter 28 Immunoprecipitation-western blot for proteins of low abundance.
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    Chapter 29 Native electrophoresis and western blot analysis: method and applications.
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    Chapter 30 Grid-immunoblotting.
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    Chapter 31 Sequential use of immunoblots for characterization of autoantibody specificities.
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    Chapter 32 Renaturation of recombinant Ro 60 autoantigen by calcium ions on PVDF membrane.
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    Chapter 33 Intermittent microwave irradiation facilitates antigen-antibody reaction in Western blot analysis.
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    Chapter 34 Detection of protein-protein interactions by far-western blotting.
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    Chapter 35 On-membrane tryptic digestion of proteins for mass spectrometry analysis.
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    Chapter 36 Western blot analysis of adhesive interactions under fluid shear conditions: the blot rolling assay.
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    Chapter 37 ELISPOT assay on membrane microplates.
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    Chapter 38 A Brief Review of Other Notable Protein Blotting Methods
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    Chapter 39 Phosphoprotein detection on protein electroblot using a phosphate-specific fluorophore.
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    Chapter 40 Rapid, antibody-free detection of recombinant proteins on blots using enzyme fragment complementation.
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    Chapter 41 Fluorescent labeling of proteins and its application to SDS-PAGE and western blotting.
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    Chapter 42 Whole and strip nitrocellulose membrane as well as a new-line-immunoblotting of antigen using the chemiluminescence technique.
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    Chapter 43 Protein stains to detect antigen on membranes.
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    Chapter 44 Use of non-radioactive detection method for north- and southwestern blot.
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    Chapter 45 Immunoblotting using radiolabeled reagents for detection.
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    Chapter 46 Detection of protein carbonyls by means of biotin hydrazide-streptavidin affinity methods.
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    Chapter 47 "Rainbow" western blotting.
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    Chapter 48 Multiple antigen detection (MAD) western blotting.
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    Chapter 49 Detection of calcium binding by Ro 60 multiple antigenic peptides on nitrocellulose membrane using Quin-2-.
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    Chapter 50 Detection of La/SS-B by western blot using nanogold-tagged antibodies and silver enhancement.
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    Chapter 51 Imaging systems for westerns: chemiluminescence vs. infrared detection.
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    Chapter 52 A fluorescent codetection system for immunoblotting and proteomics through ECL-Plex and CyDye labeling.
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    Chapter 53 Visualization of unstained protein bands on PVDF.
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    Chapter 54 Improved protein detection using cold microwave technology.
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    Chapter 55 TLC blot (far-eastern blot) and its applications.
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    Chapter 56 A brief review of other notable protein detection methods on blots.
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    Chapter 57 Sending secret messages on nitrocellulose membrane and the use of a molecular pen for orientation in ECL membrane assays.
Overall attention for this book and its chapters
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (65th percentile)
  • Good Attention Score compared to outputs of the same age and source (72nd percentile)

Mentioned by

1 Facebook page
2 Wikipedia pages


9 Dimensions

Readers on

380 Mendeley
4 CiteULike
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Protein Blotting and Detection
Published by
Methods in molecular biology, January 2009
DOI 10.1007/978-1-59745-542-8
978-1-934115-73-2, 978-1-59745-542-8, 978-9-78-193411-7

Kurien, Biji T, Scofield, R. Hal


Biji T. Kurien, R. Hal Scofield

Mendeley readers

The data shown below were compiled from readership statistics for 380 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 9 2%
Portugal 4 1%
India 2 <1%
Switzerland 2 <1%
Malaysia 2 <1%
Denmark 2 <1%
Germany 2 <1%
Australia 2 <1%
United Kingdom 2 <1%
Other 16 4%
Unknown 337 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 111 29%
Researcher 86 23%
Student > Bachelor 47 12%
Student > Master 44 12%
Student > Doctoral Student 23 6%
Other 57 15%
Unknown 12 3%
Readers by discipline Count As %
Agricultural and Biological Sciences 214 56%
Biochemistry, Genetics and Molecular Biology 54 14%
Medicine and Dentistry 25 7%
Chemistry 18 5%
Engineering 11 3%
Other 32 8%
Unknown 26 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2017.
All research outputs
of 12,167,359 outputs
Outputs from Methods in molecular biology
of 8,297 outputs
Outputs of similar age
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Outputs of similar age from Methods in molecular biology
of 11 outputs
Altmetric has tracked 12,167,359 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 8,297 research outputs from this source. They receive a mean Attention Score of 2.1. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 260,652 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.