↓ Skip to main content

Protein Blotting and Detection

Overview of attention for book
Cover of 'Protein Blotting and Detection'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Origins of protein blotting.
  3. Altmetric Badge
    Chapter 2 Protein Blotting and Detection
  4. Altmetric Badge
    Chapter 3 Introduction to protein blotting.
  5. Altmetric Badge
    Chapter 4 Simultaneous immunoblotting analysis with activity gel electrophoresis and 2-D gel electrophoresis.
  6. Altmetric Badge
    Chapter 5 Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support.
  7. Altmetric Badge
    Chapter 6 Tryptic peptide purification using polyvinylidene difluoride membrane for matrix-assisted laser desorption ionization time of flight mass spectrometry.
  8. Altmetric Badge
    Chapter 7 Affinity immunoblotting for analysis of antibody clonotype distribution in a lupus patient developing anti-Ro 60 over time.
  9. Altmetric Badge
    Chapter 8 Nonelectrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple antigens to obtain 12 immunoblots.
  10. Altmetric Badge
    Chapter 9 Slice blotting.
  11. Altmetric Badge
    Chapter 10 Localizing proteins by tissue printing.
  12. Altmetric Badge
    Chapter 11 Dot-immunobinding assay.
  13. Altmetric Badge
    Chapter 12 Capillary blotting of glycosaminoglycans on nitrocellulose membranes after agarose-gel electrophoresis separation.
  14. Altmetric Badge
    Chapter 13 Quantitative computerized western blotting.
  15. Altmetric Badge
    Chapter 14 Cationic electrophoresis and eastern blotting.
  16. Altmetric Badge
    Chapter 15 A miniaturized blotting system for simultaneous detecting of different autoantibodies.
  17. Altmetric Badge
    Chapter 16 Transfer and multiplex immunoblotting of a paraffin embedded tissue.
  18. Altmetric Badge
    Chapter 17 Multistrip western blotting to increase quantitative data output.
  19. Altmetric Badge
    Chapter 18 Western Blotting/Edman Sequencing using PVDF Membrane
  20. Altmetric Badge
    Chapter 19 Blotting from PhastGel to membranes by ultrasound.
  21. Altmetric Badge
    Chapter 20 Ultrarapid electrophoretic transfer of high and low molecular weight proteins using heat.
  22. Altmetric Badge
    Chapter 21 Strip immunoblotting of multiple antigenic peptides to nitrocellulose membrane.
  23. Altmetric Badge
    Chapter 22 Affinity purification of autoantibodies from an antigen strip excised from a nitrocellulose protein blot.
  24. Altmetric Badge
    Chapter 23 Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures.
  25. Altmetric Badge
    Chapter 24 Efficient electroblotting of very large proteins using a vertical agarose electrophoresis system.
  26. Altmetric Badge
    Chapter 25 Semi-dry protein transfer and immunodetection of P-selectin using an antibody to its C-terminal tag.
  27. Altmetric Badge
    Chapter 26 The multiple antigen blot assay: a simple, versatile and multipurpose immunoenzymatic technique.
  28. Altmetric Badge
    Chapter 27 Blotting from immobilized pH gradient gels: application to total cell lysates.
  29. Altmetric Badge
    Chapter 28 Immunoprecipitation-western blot for proteins of low abundance.
  30. Altmetric Badge
    Chapter 29 Native electrophoresis and western blot analysis: method and applications.
  31. Altmetric Badge
    Chapter 30 Grid-immunoblotting.
  32. Altmetric Badge
    Chapter 31 Sequential use of immunoblots for characterization of autoantibody specificities.
  33. Altmetric Badge
    Chapter 32 Renaturation of recombinant Ro 60 autoantigen by calcium ions on PVDF membrane.
  34. Altmetric Badge
    Chapter 33 Intermittent microwave irradiation facilitates antigen-antibody reaction in Western blot analysis.
  35. Altmetric Badge
    Chapter 34 Detection of protein-protein interactions by far-western blotting.
  36. Altmetric Badge
    Chapter 35 On-membrane tryptic digestion of proteins for mass spectrometry analysis.
  37. Altmetric Badge
    Chapter 36 Western blot analysis of adhesive interactions under fluid shear conditions: the blot rolling assay.
  38. Altmetric Badge
    Chapter 37 ELISPOT assay on membrane microplates.
  39. Altmetric Badge
    Chapter 38 A Brief Review of Other Notable Protein Blotting Methods
  40. Altmetric Badge
    Chapter 39 Phosphoprotein detection on protein electroblot using a phosphate-specific fluorophore.
  41. Altmetric Badge
    Chapter 40 Rapid, antibody-free detection of recombinant proteins on blots using enzyme fragment complementation.
  42. Altmetric Badge
    Chapter 41 Fluorescent labeling of proteins and its application to SDS-PAGE and western blotting.
  43. Altmetric Badge
    Chapter 42 Whole and strip nitrocellulose membrane as well as a new-line-immunoblotting of antigen using the chemiluminescence technique.
  44. Altmetric Badge
    Chapter 43 Protein stains to detect antigen on membranes.
  45. Altmetric Badge
    Chapter 44 Use of non-radioactive detection method for north- and southwestern blot.
  46. Altmetric Badge
    Chapter 45 Immunoblotting using radiolabeled reagents for detection.
  47. Altmetric Badge
    Chapter 46 Detection of protein carbonyls by means of biotin hydrazide-streptavidin affinity methods.
  48. Altmetric Badge
    Chapter 47 "Rainbow" western blotting.
  49. Altmetric Badge
    Chapter 48 Multiple antigen detection (MAD) western blotting.
  50. Altmetric Badge
    Chapter 49 Detection of calcium binding by Ro 60 multiple antigenic peptides on nitrocellulose membrane using Quin-2-.
  51. Altmetric Badge
    Chapter 50 Detection of La/SS-B by western blot using nanogold-tagged antibodies and silver enhancement.
  52. Altmetric Badge
    Chapter 51 Imaging systems for westerns: chemiluminescence vs. infrared detection.
  53. Altmetric Badge
    Chapter 52 A fluorescent codetection system for immunoblotting and proteomics through ECL-Plex and CyDye labeling.
  54. Altmetric Badge
    Chapter 53 Visualization of unstained protein bands on PVDF.
  55. Altmetric Badge
    Chapter 54 Improved protein detection using cold microwave technology.
  56. Altmetric Badge
    Chapter 55 TLC blot (far-eastern blot) and its applications.
  57. Altmetric Badge
    Chapter 56 A brief review of other notable protein detection methods on blots.
  58. Altmetric Badge
    Chapter 57 Sending secret messages on nitrocellulose membrane and the use of a molecular pen for orientation in ECL membrane assays.
Attention for Chapter 4: Simultaneous immunoblotting analysis with activity gel electrophoresis and 2-D gel electrophoresis.
Altmetric Badge

Citations

dimensions_citation
17 Dimensions

Readers on

mendeley
3 Mendeley
citeulike
1 CiteULike
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Simultaneous immunoblotting analysis with activity gel electrophoresis and 2-D gel electrophoresis.
Chapter number 4
Book title
Protein Blotting and Detection
Published in
Methods in molecular biology, February 2009
DOI 10.1007/978-1-59745-542-8_4
Pubmed ID
Book ISBNs
978-1-934115-73-2, 978-1-59745-542-8
Authors

Der-Yen Lee, Geen-Dong Chang

Editors

Biji T. Kurien, R. Hal Scofield

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 33%
Student > Bachelor 1 33%
Unknown 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Medicine and Dentistry 1 33%
Unknown 1 33%