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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Efficacy and immunogenicity of unmodified and pseudouridine-modified mRNA delivered systemically with lipid nanoparticles in vivo
|
|---|---|
| Published in |
Clinical Materials, September 2016
|
| DOI | 10.1016/j.biomaterials.2016.09.006 |
| Pubmed ID | |
| Authors |
Kevin J. Kauffman, Faryal F. Mir, Siddharth Jhunjhunwala, James C. Kaczmarek, Juan E. Hurtado, Jung H. Yang, Matthew J. Webber, Piotr S. Kowalski, Michael W. Heartlein, Frank DeRosa, Daniel G. Anderson |
| Abstract |
mRNA has broad potential for treating diseases requiring protein expression. However, mRNA can also induce an immune response with associated toxicity. Replacement of uridine bases with pseudouridine has been postulated to modulate both mRNA immunogenicity and potency. Here, we explore the immune response and activity of lipid nanoparticle-formulated unmodified and pseudouridine-modified mRNAs administered systemically in vivo. Pseudouridine modification to mRNA had no significant effect on lipid nanoparticle physical properties, protein expression in vivo, or mRNA immunogenicity compared to unmodified mRNA when delivered systemically with liver-targeting lipid nanoparticles, but reduced in vitro transfection levels. Indicators of a transient, extracellular innate immune response to mRNA were observed, including neutrophilia, myeloid cell activation, and up-regulation of four serum cytokines. This study provides insight into the immune responses to mRNA lipid nanoparticles, and suggests that pseudouridine modifications may be unnecessary for therapeutic application of mRNA in the liver. |
X Demographics
The data shown below were collected from the profiles of 128 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 21 | 16% |
| United States | 4 | 3% |
| Australia | 2 | 2% |
| Canada | 1 | <1% |
| Switzerland | 1 | <1% |
| Belgium | 1 | <1% |
| Madagascar | 1 | <1% |
| Comoros | 1 | <1% |
| Unknown | 96 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 120 | 94% |
| Scientists | 5 | 4% |
| Practitioners (doctors, other healthcare professionals) | 2 | 2% |
| Science communicators (journalists, bloggers, editors) | 1 | <1% |
Mendeley readers
The data shown below were compiled from readership statistics for 238 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Turkey | 1 | <1% |
| Unknown | 237 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 49 | 21% |
| Researcher | 34 | 14% |
| Student > Bachelor | 25 | 11% |
| Student > Master | 23 | 10% |
| Other | 13 | 5% |
| Other | 29 | 12% |
| Unknown | 65 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 57 | 24% |
| Agricultural and Biological Sciences | 29 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 16 | 7% |
| Immunology and Microbiology | 14 | 6% |
| Engineering | 11 | 5% |
| Other | 40 | 17% |
| Unknown | 71 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 89. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2024.
All research outputs
#485,776
of 25,654,566 outputs
Outputs from Clinical Materials
#81
of 10,810 outputs
Outputs of similar age
#9,257
of 331,615 outputs
Outputs of similar age from Clinical Materials
#2
of 117 outputs
Altmetric has tracked 25,654,566 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,810 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.9. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 331,615 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 117 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.