Title |
Rigidity of silicone substrates controls cell spreading and stem cell differentiation
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Published in |
Scientific Reports, September 2016
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DOI | 10.1038/srep33411 |
Pubmed ID | |
Authors |
Grigory Vertelov, Edgar Gutierrez, Sin-Ae Lee, Edward Ronan, Alex Groisman, Eugene Tkachenko |
Abstract |
The dependences of spreading and differentiation of stem cells plated on hydrogel and silicone gel substrates on the rigidity and porosity of the substrates have recently been a subject of some controversy. In experiments on human mesenchymal stem cells plated on soft, medium rigidity, and hard silicone gels we show that harder gels are more osteogenic, softer gels are more adipogenic, and cell spreading areas increase with the silicone gel substrate rigidity. The results of our study indicate that substrate rigidity induces some universal cellular responses independently of the porosity or topography of the substrate. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 59 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 22 | 37% |
Student > Doctoral Student | 7 | 12% |
Student > Master | 4 | 7% |
Student > Bachelor | 3 | 5% |
Professor | 2 | 3% |
Other | 5 | 8% |
Unknown | 16 | 27% |
Readers by discipline | Count | As % |
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Engineering | 11 | 19% |
Biochemistry, Genetics and Molecular Biology | 8 | 14% |
Agricultural and Biological Sciences | 6 | 10% |
Medicine and Dentistry | 3 | 5% |
Physics and Astronomy | 3 | 5% |
Other | 8 | 14% |
Unknown | 20 | 34% |