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Control of substrate access to the active site in methane monooxygenase

Overview of attention for article published in Nature, February 2013
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (92nd percentile)
  • Average Attention Score compared to outputs of the same age and source

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1 news outlet
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1 X user
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8 patents

Citations

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119 Dimensions

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242 Mendeley
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Title
Control of substrate access to the active site in methane monooxygenase
Published in
Nature, February 2013
DOI 10.1038/nature11880
Pubmed ID
Authors

Seung Jae Lee, Michael S. McCormick, Stephen J. Lippard, Uhn-Soo Cho

Abstract

Methanotrophs consume methane as their major carbon source and have an essential role in the global carbon cycle by limiting escape of this greenhouse gas to the atmosphere. These bacteria oxidize methane to methanol by soluble and particulate methane monooxygenases (MMOs). Soluble MMO contains three protein components, a 251-kilodalton hydroxylase (MMOH), a 38.6-kilodalton reductase (MMOR), and a 15.9-kilodalton regulatory protein (MMOB), required to couple electron consumption with substrate hydroxylation at the catalytic diiron centre of MMOH. Until now, the role of MMOB has remained ambiguous owing to a lack of atomic-level information about the MMOH-MMOB (hereafter termed H-B) complex. Here we remedy this deficiency by providing a crystal structure of H-B, which reveals the manner by which MMOB controls the conformation of residues in MMOH crucial for substrate access to the active site. MMOB docks at the α(2)β(2) interface of α(2)β(2)γ(2) MMOH, and triggers simultaneous conformational changes in the α-subunit that modulate oxygen and methane access as well as proton delivery to the diiron centre. Without such careful control by MMOB of these substrate routes to the diiron active site, the enzyme operates as an NADH oxidase rather than a monooxygenase. Biological catalysis involving small substrates is often accomplished in nature by large proteins and protein complexes. The structure presented in this work provides an elegant example of this principle.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 242 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 5 2%
Czechia 2 <1%
Netherlands 2 <1%
Korea, Republic of 1 <1%
India 1 <1%
Hungary 1 <1%
United Kingdom 1 <1%
Germany 1 <1%
Canada 1 <1%
Other 1 <1%
Unknown 226 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 69 29%
Researcher 38 16%
Student > Bachelor 35 14%
Student > Master 30 12%
Student > Doctoral Student 10 4%
Other 30 12%
Unknown 30 12%
Readers by discipline Count As %
Chemistry 79 33%
Agricultural and Biological Sciences 49 20%
Biochemistry, Genetics and Molecular Biology 27 11%
Engineering 15 6%
Chemical Engineering 15 6%
Other 19 8%
Unknown 38 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 16. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 July 2023.
All research outputs
#1,934,386
of 22,696,971 outputs
Outputs from Nature
#40,491
of 90,709 outputs
Outputs of similar age
#21,058
of 286,369 outputs
Outputs of similar age from Nature
#616
of 954 outputs
Altmetric has tracked 22,696,971 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 90,709 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 99.2. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 286,369 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 954 others from the same source and published within six weeks on either side of this one. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.