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Capillary Electrophoresis

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Cover of 'Capillary Electrophoresis'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 The CE-Way of Thinking: "All Is Relative!".
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    Chapter 2 A Semiempirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free Zone Electrophoresis.
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    Chapter 3 Derivatization in Capillary Electrophoresis.
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    Chapter 4 Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS Peptide Analysis: Strategies and Implementation.
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    Chapter 5 Micellar Electrokinetic Chromatography of Aminoglycosides.
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    Chapter 6 Microemulsion Electrokinetic Chromatography.
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    Chapter 7 Nonaqueous Capillary Electrophoresis Mass Spectrometry.
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    Chapter 8 Ionic Liquids in Capillary Electrophoresis.
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    Chapter 9 CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve.
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    Chapter 10 Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry.
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    Chapter 11 Use of CE to Analyze Solutes in Pico- and Nano-Liter Samples from Plant Cells and Rhizosphere.
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    Chapter 12 Analysis of Small Ions with Capillary Electrophoresis.
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    Chapter 13 Metal Ions Analysis with Capillary Zone Electrophoresis.
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    Chapter 14 Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis.
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    Chapter 15 Enantiomer Separations by Capillary Electrophoresis.
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    Chapter 16 Capillary Electrophoresis of Mono- and Oligosaccharides.
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    Chapter 17 Use of Capillary Electrophoresis for Polysaccharide Studies and Applications.
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    Chapter 18 Separation of Peptides by Capillary Electrophoresis.
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    Chapter 19 Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae Using Capillary Electrophoresis Approach.
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    Chapter 20 Capillary Electrophoretic Analysis of Classical Organic Pollutants.
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    Chapter 21 Capillary Electrophoresis in Metabolomics.
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    Chapter 22 Capillary Electrophoresis in Food and Foodomics.
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    Chapter 23 Capillary Electrophoresis in Wine Science.
Attention for Chapter 1: The CE-Way of Thinking: "All Is Relative!".
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Chapter title
The CE-Way of Thinking: "All Is Relative!".
Chapter number 1
Book title
Capillary Electrophoresis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6403-1_1
Pubmed ID
Book ISBNs
978-1-4939-6401-7, 978-1-4939-6403-1
Authors

Philippe Schmitt-Kopplin, Agnes Fekete

Editors

Philippe Schmitt-Kopplin

Abstract

Over the last two decades the development of capillary electrophoresis instruments lead to systems with programmable sampler, separation column, separation buffer, and detection devices comparable visually in many aspects to the setup of classical chromatography.Two processes make capillary electrophoresis essentially different from chromatography and are the basis of the CE-way of thinking, namely, the injection type and the liquid flow within the capillary. (1) When the injection is made hydrodynamically (such as in most of the found applications in the literature), the injected volumes are directly dependent on the type and size of the separation capillary. (2) The buffer velocity is not pressure driven as in liquid chromatography but electrokinetically governed by the quality of the capillary surface (separation buffer dependant surface charge) inducing an electroosmotic flow (EOF). The EOF undergoes small variations and is not necessarily identical from one separation or day to the other. The direct consequence is an apparent nonreproducible migration time of the analytes, even though the own velocity of the ions is the same.The effective mobility (field strength normalized velocity) of the ions is a possible parameterization from acquired timescale to effective mobility-scale electropherograms leading to a reproducible visualization and better quantification with a direct relation to structural characters of the analytes (i.e., charge and size-see chapter on semiempirical modelization).

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 17%
Unknown 5 83%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 67%
Researcher 1 17%
Unknown 1 17%
Readers by discipline Count As %
Chemistry 4 67%
Chemical Engineering 1 17%
Unknown 1 17%