Chapter title |
Micellar Electrokinetic Chromatography of Aminoglycosides.
|
---|---|
Chapter number | 5 |
Book title |
Capillary Electrophoresis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6403-1_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6401-7, 978-1-4939-6403-1
|
Authors |
Ulrike Holzgrabe, Stefanie Schmitt, Frank Wienen |
Editors |
Philippe Schmitt-Kopplin |
Abstract |
The components of the aminoglycosides, e.g., gentamicin, sisomicin, netilmicin, kanamycin, amikacin, and tobramycin, and related impurities of these antibiotics can be separated by means of micellar electrokinetic chromatography (MEKC). Derivatization with o-phthaldialdehyde and thioglycolic acid is found to be appropriate for these antibiotics. The background electrolyte was composed of sodium tetraborate (100 mM), sodium deoxycholate (20 mM), and β-cyclodextrin (15 mM) having a pH value of 10.0. This method is valid for evaluation of gentamicin, kanamycin, and tobramycin. It has to be adopted for amikacin, paromomycin, neomycin, and netilmicin. |
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