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Capillary Electrophoresis

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Cover of 'Capillary Electrophoresis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The CE-Way of Thinking: "All Is Relative!".
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    Chapter 2 A Semiempirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free Zone Electrophoresis.
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    Chapter 3 Derivatization in Capillary Electrophoresis.
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    Chapter 4 Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS Peptide Analysis: Strategies and Implementation.
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    Chapter 5 Micellar Electrokinetic Chromatography of Aminoglycosides.
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    Chapter 6 Microemulsion Electrokinetic Chromatography.
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    Chapter 7 Nonaqueous Capillary Electrophoresis Mass Spectrometry.
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    Chapter 8 Ionic Liquids in Capillary Electrophoresis.
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    Chapter 9 CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve.
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    Chapter 10 Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry.
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    Chapter 11 Use of CE to Analyze Solutes in Pico- and Nano-Liter Samples from Plant Cells and Rhizosphere.
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    Chapter 12 Analysis of Small Ions with Capillary Electrophoresis.
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    Chapter 13 Metal Ions Analysis with Capillary Zone Electrophoresis.
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    Chapter 14 Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis.
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    Chapter 15 Enantiomer Separations by Capillary Electrophoresis.
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    Chapter 16 Capillary Electrophoresis of Mono- and Oligosaccharides.
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    Chapter 17 Use of Capillary Electrophoresis for Polysaccharide Studies and Applications.
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    Chapter 18 Separation of Peptides by Capillary Electrophoresis.
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    Chapter 19 Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae Using Capillary Electrophoresis Approach.
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    Chapter 20 Capillary Electrophoretic Analysis of Classical Organic Pollutants.
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    Chapter 21 Capillary Electrophoresis in Metabolomics.
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    Chapter 22 Capillary Electrophoresis in Food and Foodomics.
  24. Altmetric Badge
    Chapter 23 Capillary Electrophoresis in Wine Science.
Attention for Chapter 6: Microemulsion Electrokinetic Chromatography.
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Chapter title
Microemulsion Electrokinetic Chromatography.
Chapter number 6
Book title
Capillary Electrophoresis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6403-1_6
Pubmed ID
Book ISBNs
978-1-4939-6401-7, 978-1-4939-6403-1
Authors

Wolfgang Buchberger

Editors

Philippe Schmitt-Kopplin

Abstract

Microemulsion electrokinetic chromatography (MEEKC) is a special mode of capillary electrophoresis employing a microemulsion as carrier electrolyte. Analytes may partition between the aqueous phase of the microemulsion and its oil droplets which act as a pseudostationary phase. The technique is well suited for the separation of neutral species, in which case charged oil droplets (obtained by addition of an anionic or cationic surfactant) are present. A single set of separation parameters may be sufficient for separation of a wide range of analytes belonging to quite different chemical classes. Fine-tuning of resolution and analysis time may be achieved by addition of organic solvents, by changes in the nature of the surfactants (and cosurfactants) used to stabilize the microemulsion, or by various additives that may undergo some additional interactions with the analytes. Besides the separation of neutral analytes (which may be the most important application area of MEEKC), it can also be employed for cationic and/or anionic species. In this chapter, MEEKC conditions are summarized that have proven their reliability for routine analysis. Furthermore, the mechanisms encountered in MEEKC allow an efficient on-capillary preconcentration of analytes, so that the problem of poor concentration sensitivity of ultraviolet absorbance detection is circumvented.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Belgium 1 6%
Unknown 15 94%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 25%
Professor 3 19%
Professor > Associate Professor 2 13%
Student > Ph. D. Student 2 13%
Lecturer 1 6%
Other 3 19%
Unknown 1 6%
Readers by discipline Count As %
Chemistry 8 50%
Agricultural and Biological Sciences 3 19%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Medicine and Dentistry 1 6%
Unknown 3 19%