Chapter title |
CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve.
|
---|---|
Chapter number | 9 |
Book title |
Capillary Electrophoresis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6403-1_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6401-7, 978-1-4939-6403-1
|
Authors |
Felix J. Kohl, Christian Neusüß |
Editors |
Philippe Schmitt-Kopplin |
Abstract |
The hyphenation of capillary electrophoresis and electrospray ionization-mass spectrometry is a powerful tool for peptide and protein analysis. It provides high separation power in combination with sensitive and selective detection and the possibility of analyte identification. Unfortunately, many proven capillary electrophoresis methods are not compatible with electrospray-mass spectrometry since several compounds of best separating background electrolytes are interfering in the electrospray ionization. Here, we describe a two-dimensional capillary electrophoresis system using the second dimension as a cleanup stage in order to remove interfering compounds to enable electrospray-mass spectrometry coupling. |
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