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Constitutive β-Catenin Overexpression Represses Lncrna MIR100HG Transcription via HDAC6-Mediated Histone Modification in Colorectal Cancer

Overview of attention for article published in Molecular Cancer Research, March 2022
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Title
Constitutive β-Catenin Overexpression Represses Lncrna MIR100HG Transcription via HDAC6-Mediated Histone Modification in Colorectal Cancer
Published in
Molecular Cancer Research, March 2022
DOI 10.1158/1541-7786.mcr-21-0923
Pubmed ID
Authors

Jian Peng, Yiming Ma, Xinhua Zhao, Xu Yang, Hongying Wang

Abstract

Wnt/β-catenin signaling plays a critical role in colonic carcinogenesis. However, non-coding RNAs (ncRNAs) transcriptionally regulated by β-catenin are largely unknown. Herein, we found that lncRNA MIR100HG (lnc-MIR100HG) negatively correlated with target genes of β-catenin from The Cancer Genome Atlas colorectal carcinoma (CRC) database, which was verified in 48 paired CRC specimens. In addition, constitutive overexpression of β-catenin decreased primary and mature lnc-MIR100HG levels, while blockage of β-catenin activity with siRNA or inhibitors significantly increased their expression. DNA pull-down and chromatin immunoprecipitation revealed the binding of β-catenin/TCF4 to the MIR100HG promoter. Moreover, β-catenin forced expression reduced the enrichment of H3K27Ac, an active transcription marker, on the promoter, while β-catenin inhibition reversed this effect. Furthermore, HDAC6 was recruited to the MIR100HG promoter and downregulated H3K27Ac enrichment in a β-catenin-dependent manner. Besides, HDAC6 was upregulated and negatively correlated with lnc-MIR100HG in CRC specimens. Functional studies showed that lnc-MIR100HG overexpression induced cell cycle G0/G1 arrest and repressed cell proliferation via p57 upregulation in vitro and in vivo. Taken together, we found that ectopic β-catenin transcriptionally repressed lnc-MIR100HG expression through HDAC6-mediated histone modification in CRC. Lnc-MIR100HG regulates the cell cycle through p57. Implications: It provides a novel downstream mechanism highlighting β-catenin action during colon carcinogenesis and may shed light for further therapeutic approaches.

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Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 June 2022.
All research outputs
#13,355,748
of 21,423,731 outputs
Outputs from Molecular Cancer Research
#1,100
of 1,798 outputs
Outputs of similar age
#168,101
of 336,940 outputs
Outputs of similar age from Molecular Cancer Research
#29
of 36 outputs
Altmetric has tracked 21,423,731 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,798 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.9. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
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We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.