| Chapter title |
Application of Proteomics in Cancer Biomarker Discovery: GeLC-MS/MS
|
|---|---|
| Chapter number | 11 |
| Book title |
Cancer Gene Profiling
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3204-7_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3203-0, 978-1-4939-3204-7
|
| Authors |
Pedro R. Cutillas, Tatjana Crnogorac-Jurcevic |
| Editors |
Robert Grützmann, Christian Pilarsky |
| Abstract |
Proteomic approaches are being increasingly applied to study multiple facets of healthy and diseased processes. In particular, the application of global proteome profiling in the field of oncology is already starting to shape the diagnostic, prognostic, monitoring, and therapeutic approaches. At the heart of such approaches lies a quest for clinically relevant biomarkers, particularly arising from global analyses of body fluids, as, in major part, they represent easily accessible and noninvasive matrices. A detailed protocol of one of the popular approaches for global proteome profiling, SDS-PAGE-liquid chromatography-tandem mass spectrometry or GeLC-MS/MS, and its application for biomarker discovery in urine is provided here. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 25% |
| Student > Ph. D. Student | 1 | 25% |
| Student > Bachelor | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 25% |
| Agricultural and Biological Sciences | 1 | 25% |
| Medicine and Dentistry | 1 | 25% |
| Unknown | 1 | 25% |