↓ Skip to main content


Overview of attention for book
Cover of 'Drosophila'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Using FlyBase, a Database of Drosophila Genes and Genomes.
  3. Altmetric Badge
    Chapter 2 The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression.
  4. Altmetric Badge
    Chapter 3 The Q-System: A Versatile Expression System for Drosophila.
  5. Altmetric Badge
    Chapter 4 Analysis of MicroRNA Function in Drosophila.
  6. Altmetric Badge
    Chapter 5 Methods for High-Throughput RNAi Screening in Drosophila Cells.
  7. Altmetric Badge
    Chapter 6 A Guide to Genome-Wide In Vivo RNAi Applications in Drosophila.
  8. Altmetric Badge
    Chapter 7 Creating Heritable Mutations in Drosophila with CRISPR-Cas9.
  9. Altmetric Badge
    Chapter 8 Performing Chromophore-Assisted Laser Inactivation in Drosophila Embryos Using GFP.
  10. Altmetric Badge
    Chapter 9 deGradFP: A System to Knockdown GFP-Tagged Proteins.
  11. Altmetric Badge
    Chapter 10 Drosophila
  12. Altmetric Badge
    Chapter 11 Cultivation and Live Imaging of Drosophila Imaginal Discs.
  13. Altmetric Badge
    Chapter 12 Cultivation and Live Imaging of Drosophila Ovaries.
  14. Altmetric Badge
    Chapter 13 Segmentation and Quantitative Analysis of Epithelial Tissues.
  15. Altmetric Badge
    Chapter 14 Laser Ablation to Probe the Epithelial Mechanics in Drosophila.
  16. Altmetric Badge
    Chapter 15 Rapid Ovary Mass-Isolation (ROMi) to Obtain Large Quantities of Drosophila Egg Chambers for Fluorescent In Situ Hybridization.
  17. Altmetric Badge
    Chapter 16 Drosophila
  18. Altmetric Badge
    Chapter 17 Protocols to Study Growth and Metabolism in Drosophila.
  19. Altmetric Badge
    Chapter 18 Protocols to Study Aging in Drosophila.
  20. Altmetric Badge
    Chapter 19 Protocols to Study Behavior in Drosophila.
  21. Altmetric Badge
    Chapter 20 Electrophysiological Recordings from Lobula Plate Tangential Cells in Drosophila.
  22. Altmetric Badge
    Chapter 21 Methods to Establish Drosophila Cell Lines.
  23. Altmetric Badge
    Chapter 22 Erratum to: The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression
Attention for Chapter 9: deGradFP: A System to Knockdown GFP-Tagged Proteins.
Altmetric Badge

About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#23 of 9,313)
  • High Attention Score compared to outputs of the same age (95th percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Mentioned by

2 blogs
59 tweeters
2 Facebook pages


2 Dimensions

Readers on

46 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
deGradFP: A System to Knockdown GFP-Tagged Proteins.
Chapter number 9
Book title
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6371-3_9
Pubmed ID
Book ISBNs
978-1-4939-6369-0, 978-1-4939-6371-3

Emmanuel Caussinus, Markus Affolter, Caussinus, Emmanuel, Affolter, Markus, Anthony L. Komaroff, Komaroff, Anthony L.


Christian Dahmann


Protein depletion by genetic means, in a very general sense including the use of RNA interference [1, 2] or CRISPR/Cas9-based methods, represents a central paradigm of modern biology to study protein functions in vivo. However, acting upstream the proteic level is a limiting factor if the turnover of the target protein is slow or the existing pool of the target protein is important (for instance, in insect embryos, as a consequence of a strong maternal contribution). In order to circumvent these problems, we developed deGradFP [3, 4]. deGradFP harnesses the ubiquitin-proteasome pathway to achieve direct depletion of GFP-tagged proteins. deGradFP is in essence a universal method because it relies on an evolutionarily conserved machinery for protein catabolism in eukaryotic cells; see refs. 5, 6 for review. deGradFP is particularly convenient in Drosophila melanogaster where it is implemented by a genetically encoded effector expressed under the control of the Gal4 system. deGradFP is a ready-to-use solution to perform knockdowns at the protein level if a fly line carrying a functional GFP-tagged version of the gene of interest is available. Many such lines have already been generated by the Drosophila community through different technologies allowing to make genomic rescue constructs or direct GFP knockins: protein-trap stock collections [7, 8] ( http://cooley.medicine.yale.edu/flytrap/ , http://www.flyprot.org/ ), P[acman] system [9], MiMIC lines [10, 11], and CRISPR/Cas9-driven homologous recombination.Two essential controls of a protein knockdown experiment are easily achieved using deGradFP. First, the removal of the target protein can be assessed by monitoring the disappearance of the GFP tag by fluorescence microscopy in parallel to the documentation of the phenotype of the protein knockdown (see Note 1 ). Second, the potential nonspecific effects of deGradFP can be assessed in control fly lacking a GFP-tagged target protein. So far, no nonspecific effects of the deGradFP effector have been reported [3].

Twitter Demographics

The data shown below were collected from the profiles of 59 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 46 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 24%
Student > Ph. D. Student 9 20%
Student > Master 8 17%
Student > Bachelor 3 7%
Other 2 4%
Other 5 11%
Unknown 8 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 35%
Biochemistry, Genetics and Molecular Biology 16 35%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Immunology and Microbiology 2 4%
Chemical Engineering 1 2%
Other 2 4%
Unknown 7 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 57. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 May 2020.
All research outputs
of 16,046,105 outputs
Outputs from Methods in molecular biology
of 9,313 outputs
Outputs of similar age
of 272,367 outputs
Outputs of similar age from Methods in molecular biology
of 8 outputs
Altmetric has tracked 16,046,105 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,313 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 272,367 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them