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Mendeley readers
| Chapter title |
Cultivation and Live Imaging of Drosophila Imaginal Discs.
|
|---|---|
| Chapter number | 11 |
| Book title |
Drosophila
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6371-3_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6369-0, 978-1-4939-6371-3
|
| Authors |
Simon Restrepo, Jeremiah J. Zartman, Konrad Basler |
| Editors |
Christian Dahmann |
| Abstract |
The ex vivo cultivation and live imaging of wing discs open exciting new research avenues by overcoming the limitations of end-point analysis of fixed tissues. Here we describe how to prepare an optimized wing disc culture medium (WM1) and how to dissect and arrange wing discs for cultivation and live imaging. This protocol enables the study of dynamic phenomena such as cell division and delamination as well as the use of pharmacological compounds and biosensors. Wing discs cultured and imaged as described here, maintain constant levels of proliferation during the first ten hours of culture. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 29% |
| Researcher | 3 | 18% |
| Student > Bachelor | 2 | 12% |
| Professor | 1 | 6% |
| Student > Master | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 4 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 41% |
| Agricultural and Biological Sciences | 5 | 29% |
| Immunology and Microbiology | 1 | 6% |
| Neuroscience | 1 | 6% |
| Engineering | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 2 | 12% |