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Drosophila

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Cover of 'Drosophila'

Table of Contents

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    Book Overview
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    Chapter 1 Using FlyBase, a Database of Drosophila Genes and Genomes.
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    Chapter 2 The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression.
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    Chapter 3 The Q-System: A Versatile Expression System for Drosophila.
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    Chapter 4 Analysis of MicroRNA Function in Drosophila.
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    Chapter 5 Methods for High-Throughput RNAi Screening in Drosophila Cells.
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    Chapter 6 A Guide to Genome-Wide In Vivo RNAi Applications in Drosophila.
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    Chapter 7 Creating Heritable Mutations in Drosophila with CRISPR-Cas9.
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    Chapter 8 Performing Chromophore-Assisted Laser Inactivation in Drosophila Embryos Using GFP.
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    Chapter 9 deGradFP: A System to Knockdown GFP-Tagged Proteins.
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    Chapter 10 Drosophila
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    Chapter 11 Cultivation and Live Imaging of Drosophila Imaginal Discs.
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    Chapter 12 Cultivation and Live Imaging of Drosophila Ovaries.
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    Chapter 13 Segmentation and Quantitative Analysis of Epithelial Tissues.
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    Chapter 14 Laser Ablation to Probe the Epithelial Mechanics in Drosophila.
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    Chapter 15 Rapid Ovary Mass-Isolation (ROMi) to Obtain Large Quantities of Drosophila Egg Chambers for Fluorescent In Situ Hybridization.
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    Chapter 16 Drosophila
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    Chapter 17 Protocols to Study Growth and Metabolism in Drosophila.
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    Chapter 18 Protocols to Study Aging in Drosophila.
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    Chapter 19 Protocols to Study Behavior in Drosophila.
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    Chapter 20 Electrophysiological Recordings from Lobula Plate Tangential Cells in Drosophila.
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    Chapter 21 Methods to Establish Drosophila Cell Lines.
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    Chapter 22 Erratum to: The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression
Attention for Chapter 14: Laser Ablation to Probe the Epithelial Mechanics in Drosophila.
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Chapter title
Laser Ablation to Probe the Epithelial Mechanics in Drosophila.
Chapter number 14
Book title
Drosophila
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6371-3_14
Pubmed ID
Book ISBNs
978-1-4939-6369-0, 978-1-4939-6371-3
Authors

Pruthvi C. Shivakumar, Pierre-François Lenne

Editors

Christian Dahmann

Abstract

Laser ablation is nowadays a widespread technique to probe tissue mechanics during development. Here we describe the setup of one such ablation system and ablation experiments performed on the embryo and pupa of Drosophila. We describe in detail the process of sample preparation, how to disrupt single-cell junctions and perform linear or circular cuts at the tissue scale, and how to analyze the data to determine relevant mechanical parameters.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 32%
Student > Ph. D. Student 5 18%
Student > Bachelor 3 11%
Professor 1 4%
Student > Master 1 4%
Other 1 4%
Unknown 8 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 39%
Agricultural and Biological Sciences 5 18%
Physics and Astronomy 3 11%
Neuroscience 2 7%
Unknown 7 25%